Metabonomics delineates allergic reactions induced by Shuang-huang-lian injection in rats using ultra performance liquid chromatography-mass spectrometry.
10.1016/S1875-5364(18)30101-8
- Author:
Yuan-Yuan GU
1
;
Lang SHI
2
;
Dan-Dan ZHANG
1
;
Xin HUANG
2
;
Da-Zhong CHEN
3
Author Information
1. Research Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
2. First Clinical Medical College, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
3. Research Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China. Electronic address: 1259779868@qq.com.
- Publication Type:Journal Article
- Keywords:
Allergic mechanism;
Biomarker;
Metabolites;
Metabonomics;
Shuang-huang-lian injection
- MeSH:
Animals;
Biomarkers;
urine;
Chromatography, High Pressure Liquid;
Drug Hypersensitivity;
etiology;
urine;
Drugs, Chinese Herbal;
administration & dosage;
adverse effects;
Histamine;
urine;
Male;
Metabolomics;
Rats;
Rats, Sprague-Dawley;
Tandem Mass Spectrometry
- From:
Chinese Journal of Natural Medicines (English Ed.)
2018;16(8):628-640
- CountryChina
- Language:English
-
Abstract:
Shuang-huang-lian Injection (SHLI) is the first successfully developed drug from traditional Chinese medicine (TCM) powder for injection, since its use for the treatment of acute respiratory tract infection, pneumonia, influenza, etc. At the same time, its allergic reactions have also emerged, which limits clinical applications. However, few scholars pay attention to the mechanism of allergic reactions. In this present study, metabonomics technology was used to explore the changes in endogenous metabolites in urine of the rat model of SHLI induced allergic reaction; we and analyzed the metabolites, metabolic pathway, and the mechanism which were closely related to the allergic reactions. The levels of serum histamine and tryptase were examined and changes in histomorphology were also observed. Based on the UPLC-Q-TOF/MS metabonomics, we carried out the pattern recognition analysis, selected potential biomarkers associated with allergic reactions, and explored the pathological mechanism for SHLI induced allergic reaction, which laid the foundation for the safety research of SHLI. Our results showed that SHLI increased the levels of serum histamine and tryptase in rats with allergic reaction; we determined 15 biomarkers in rat allergic reaction model induced by SHLI and found multiple metabolic pathways involved, such as metabolism of linolenic acid, phenylalanine, amino acid, 2-oxo acid, and purine and other metabolic pathways.
