An HPLC-MS/MS method for the quantitative determination of platycodin D in rat plasma and its application to the pharmacokinetics of Platycodi Radix extract.
10.1016/S1875-5364(14)60026-1
- Author:
Qin ZHAN
1
;
Feng ZHANG
1
;
Shou-Hong GAO
1
;
Fei CAI
1
;
Bo JIANG
1
;
Lian-Na SUN
2
;
Wan-Sheng CHEN
3
Author Information
1. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China.
2. Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China; Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
3. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China. Electronic address: chenwanshengsmmu@aliyun.com.
- Publication Type:Journal Article
- Keywords:
HPLC-MS/MS;
Pharmacokinetics;
Platycodi Radix;
Platycodin D;
Platycodon grandiflorus;
Rat plasma
- MeSH:
Administration, Oral;
Animals;
Biological Availability;
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
pharmacokinetics;
Male;
Plant Roots;
chemistry;
Platycodon;
chemistry;
Rats;
Rats, Sprague-Dawley;
Saponins;
blood;
pharmacokinetics;
Tandem Mass Spectrometry;
methods;
Triterpenes;
blood;
pharmacokinetics
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(2):154-160
- CountryChina
- Language:English
-
Abstract:
AIMS:To develop an HPLC-MS/MS method for the quantification of platycodin D (PD) in rat plasma, and to acquire the main pharmacokinetic parameters of PD after oral administration of pure PD or of Platycodi Radix extract (PRE) containing PD.
METHOD:Plasma samples were pretreated with solid-phase extraction using an Oasis® HLB SPE cartridge. Madecassoside was used as the internal standard (IS). Chromatographic separation was achieved on an ODS column (100 mm × 2.1 mm i.d., 3.5 μm) with a mobile phase consisting of acetonitrile/water (30 : 70, V/V) containing 0.1 mmol·L(-1) ammonium acetate at a flow rate of 0.25 mL·min(-1). The detection was performed on a triple quadruple tandem mass spectrometer using an electrospray ionization (ESI) source with a chromatographic run time of 3.0 min. The detection was operated by multiple reaction monitoring (MRM) of the transitions of m/z 1 223.6→469.2 for PD and of m/z 973.6→469.2 for madecassoside (IS), respectively.
RESULTS:The calibration curve was linear from 5 to 2 000 ng·mL(-1) (r(2) >0.99) with a lower limit of quantification (LLOQ) of 5 ng·mL(-1). The intra- and inter-day precision (relative standard deviation, RSD) values were below 15% and the accuracy (relative error, RE) was from -15% to +15% at three quality control (QC) levels. Plasma concentrations of PD were determined for 24 h after i.v. administration of PD, and oral administration of PD and PRE, respectively. The absolute oral bioavailability of PD in rats was found to be (0.48 ± 0.19)% when administered PD, and to be (1.81 ± 0.89)% when administered PRE.
CONCLUSION:The developed HPLC-MS/MS method was successfully applied to assess the pharmacokinetic parameters and oral bioavailability of PD in rats after administration of PD and Platycodi Radix extract.
