Simultaneous quantitative determination of five alkaloids in Catharanthus roseus by HPLC-ESI-MS/MS.
10.1016/S1875-5364(14)60120-5
- Author:
Lin ZHANG
1
;
Qing-Hui GAI
1
;
Yuan-Gang ZU
2
,
3
;
Lei YANG
1
;
Yu-Liang MA
1
;
Yang LIU
1
Author Information
1. State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China; Engineering Research Center of Forestry Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China; Key Laboratory of Forest Plant Ecology, Ministry of Education; Northeast Forestry University, Harbin, 150040, China.
2. State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China; Engineering Research Center of Forestry Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China; Key Laboratory of Forest Plant Ecology, Ministry of Education; Northeast Forestry University, Harbin, 150040, China. Electronic address: zuyuanggang@
3. com.
- Publication Type:Journal Article
- Keywords:
Catharanthus roseus;
HPLC-ESI-MS/MS;
Monomeric and bisindole alkaloids
- MeSH:
Alkaloids;
chemistry;
Catharanthus;
chemistry;
China;
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
Tandem Mass Spectrometry;
methods
- From:
Chinese Journal of Natural Medicines (English Ed.)
2014;12(10):786-793
- CountryChina
- Language:English
-
Abstract:
AIM:To establish a method to simultaneously determine the main five alkaloids of Catharanthus roseus for trace samples, a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) analysis method was developed.
METHOD:The five Catharanthus alkaloids, vinblastine, vincristine, vinleurosine, vindoline, and catharanthine were chromatographically separated on a C18 HPLC column. The mobile phase was methanol-15 nmol·L(-1) ammonium acetate containing 0.02% formic acid (65 : 35, V/V). The quantification of these alkaloids was based on the Multiple Reaction Monitoring (MRM) mode.
RESULTS:This method was validated, and the results achieved the aims of the study. The intra- and inter-day precision and accuracy of the five alkaloids were within 1.2%-11.5% (RSD%) and -10.9%-10.5% (RE%). The recovery rates of the five alkaloids of samples were from 79.9% to 91.5%. The five analytes were stable at room temperature for 2 h, at 4 °C for 12 h, and at -20 °C for two weeks. The developed method was applied successfully to determine the content of the five alkaloids in three plant parts of three batches of C. roseus with a minute amount collected from three regions of China.
CONCLUSION:The HPLC-ESI-MS/MS method can be used for the simultaneous determination of five important alkaloids in trace C. roseus samples.
