Identification of processed Chinese medicinal materials using DNA mini-barcoding.
10.1016/S1875-5364(17)30073-0
- Author:
Ming SONG
1
;
Gang-Qiang DONG
2
;
Ya-Qin ZHANG
3
;
Xia LIU
4
,
5
;
Wei SUN
6
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China; Amway (China) Botanical Research and Development Center, Wuxi 214145, China.
2. Amway (China) Botanical Research and Development Center, Wuxi 214145, China.
3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
4. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China. Electronic address: lrx1125@
5. com.
6. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. Electronic address: wsun@icmm.ac.cn.
- Publication Type:Journal Article
- Keywords:
DNA barcoding;
Identification;
Mini-barcode;
Processed medicinal materials;
trnL (UAA) intron P6 loop
- MeSH:
DNA Barcoding, Taxonomic;
methods;
DNA, Plant;
genetics;
Discriminant Analysis;
Drugs, Chinese Herbal;
chemistry;
classification;
Introns;
Plant Proteins;
genetics;
Plants, Medicinal;
chemistry;
classification;
genetics
- From:
Chinese Journal of Natural Medicines (English Ed.)
2017;15(7):481-486
- CountryChina
- Language:English
-
Abstract:
Most of Chinese medicinal herbs are subjected to traditional processing procedures, including stir-frying, charring, steaming, boiling, and calcining before they are released into dispensaries. The marketing and identification of processed medicinal materials is a growing issue in the marketplace. However, conventional methods of identification have limitations, while DNA mini-barcoding, based on the sequencing of a short-standardized region, has received considerable attention as a new potential means to identify processed medicinal materials. In the present study, six DNA barcode loci including ITS2, psbA-trnH, rbcL, matK, trnL (UAA) intron and its P6 loop, were employed for the authentication of 45 processed samples belonging to 15 species. We evaluated the amplification efficiency of each locus. We also examined the identification accuracy of the potential mini-barcode locus, of trnL (UAA) intron P6 loop. Our results showed that the five primary barcode loci were successfully amplified in only 8.89%-20% of the processed samples, while the amplification rates of the trnL (UAA) intron P6 loop were higher, at 75.56% successful amplification. We compared the mini-barcode sequences with Genbank using the Blast program. The analysis showed that 45.23% samples could be identified to genus level, while only one sample could be identified to the species level. We conclude that trnL (UAA) p6 loop is a candidate mini-barcode that has shown its potential and may become a universal mini-barcode as complementary barcode for authenticity testing and will play an important role in medicinal materials control.
