Contamination state of Staphylococcus aureus from toilets of primary schools in Guangzhou
10.16835/j.cnki.1000-9817.2020.02.036
- VernacularTitle:广州市小学厕所环境金黄色葡萄球菌污染现状
- Author:
ZHANG Wencui , LIANG Jianping, LIN Jialing, WANG Yingying, HE Suiping, LI Ying, LIU Ning, YAO Zhenjiang
1
Author Information
1. School of Public Health, Guangdong Pharmaceutical University, Guangzhou(510310), China
- Publication Type:Journal Article
- Keywords:
Staphylococcus aureus;
Environmental pollutation;
Quality of health care;
Student health services
- From:
Chinese Journal of School Health
2020;41(2):295-297
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the contamination, antimicrobial resistance and virulence genes of S. aureus from toilets of primary schools in Guangzhou.
Methods:The surface samples of toilets were collected from eight primary schools in Guangzhou from May to July 2016. The standard microbiological assays, disk diffusion methods and PCR technique were used for the isolation and identification, antimicrobial resistance and virulence genes of S. aureus .
Results:The contamination rate of S. aureus and MRSA was 6.25% and 3.13%, respectively. There was significant difference in the contamination rate of S. aureus among different sampling sites ( χ 2=15.15, P <0.01) and the highest contamination rate was on the ground (15.00%).The most predominant antibiotic for S. aureus was penicillin (100.00%) and the proportions of resistant to teicoplanin, erythromycin,rifampicin, clindamycin and linezolid were more than 75.00%.The multidrug resistant rate of S. aureus was 85.00%.The detection rate of virulence genes of S. aureus was sea (50.00%), tst (30.00%), etb (15.00%), eta (10.00%), seb (10.00%) and pvl (5.00%), respectively.
Conclusion:The contamination rate of S. aureus from toilets of primary schools in Guangzhou is in a lower level among similar researches. However, the contamination of MRSA is serious, which accounts for half of S. aureus . In addition, S. aureus isolates show high multi-drug resistant rate and high detection rate of virulence genes.
