Diterpene ginkgolides meglumine injection attenuates oxygen-glucose deprivation-induced apoptosis of nerve cells via inhibition of calpain signaling pathway
10.11665/j.issn.1000-5048.20150612
- VernacularTitle:银杏二萜内酯葡胺注射液通过下调calpain信号通路抑制脑缺血神经细胞凋亡
- Author:
Qiu LIU
1
;
Zhiliang XU
;
Zhiquan JIN
;
Jun ZHOU
;
Yu&prime
;
an BI
;
Zhenzhong WANG
;
Wei XIAO
Author Information
1. 江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室
- Publication Type:Journal Article
- Keywords:
diterpene ginkgolides meglumine injection;
oxygen-glucose deprivation;
apoptosis;
calcium;
calpain;
caspase-12
- From:
Journal of China Pharmaceutical University
2015;46(6):707-711
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the anti-apoptotic effect of diterpene ginkgolides meglumine injection(DGMI)on SH-SY5Y cells induced by oxygen-glucose deprivation/reoxygenation(OGD/R), and to explore its mechanisms. After 4 h of OGD, the SH-SY5Y cells were treated with 25 mg/L DGMI for 1 h. The release of lactic dehydrogenase(LDH)was measured by cytotoxicity detection kitplus. Cell apoptosis was detected by caspase-3/7 assays. Cell death was detected by ELISA. The concentration of ［Ca2+］i in cytoplasm was measured by Fluo-3 AM and the levels of calpain and cleaved capaease-12 were evaluated by western blot. As we expected, DGMI significantly decreased the release of LDH, the concentration of ［Ca2+］i, the protein levels of calpain and cleaved caspase-12. Furthermore, DGMI injection also attenuated the activities of caspase-3/7 and the contents of cytoplasmic histone-associated- DNA-fragments. These data demonstrated that the DGMI injection showed good anti-apoptotic effect in SH-SY5Y cells induced by OGD/R. The mechanisms may be associated with the inhibition of Ca2+/calpain/caspase-12/caspase-3 signaling pathway.
