Inhibitory effects on glucose metabolism of human gastric carcinoma MGC-803 cells induced by wogonin
10.11665/j.issn.1000-5048.20150314
- VernacularTitle:汉黄芩素抑制人胃癌MGC-803细胞糖代谢的作用
- Author:
Qian YIN
1
;
Qinsheng DAI
;
Yikai ZHAO
;
Qinglong GUO
Author Information
1. 中国药科大学江苏省肿瘤发生与干预重点实验室；天然药物活性组分与药效国家重点实验室
- Publication Type:Journal Article
- Keywords:
wogonin;
MGC-803 cells;
glucose metabolism;
gastric carcinoma
- From:
Journal of China Pharmaceutical University
2015;46(3):345-349
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the inhibitory effect of wogonin on glucose metabolism in human gastric carcinoma MGC-803 cells and its underlying mechanism, Amplex Red Glucose/Glucose Oxidase Assay Kit was used to explore the influence of wogonin on glucose uptake of MGC-803 cells and lactate assay kit was adopted to evaluate the lactate generation of MGC-803 cells. Annexin V/PI staining assay was performed to observe the apoptotic rate of MGC-803 cells. Changes in the expression of hexokinase 2(HK II), glucose transporter 1(GLUT1), pyruvate dehydrogenase kinase(PDHK)and lactate dehydrogenase A(LDH-A)were assessed by Western blot. These results demonstrated that wogonin significantly reduced glucose uptake of MGC-803 cells at certain concentration. Meanwhile, wogonin apparently reduced the lactate generation of MGC-803 cells, without inducing notable cell apoptosis. Besides, with the impact of wogonin, the expression of glucose metabolism related proteins declined obviously. In a word, wogonin could inhibit the glucose metabolism of MGC-803 cells without inducing apparent apoptosis, which may be related to its inhibitory influence on the proteins in glucose metabolism of MGC-803 cells.
