Drug screening model of treating pigmentation disorders in tyrp1a transgenic zebrafish
10.11665/j.issn.1000-5048.20160618
- VernacularTitle:基于tyrp1a转基因斑马鱼构建色素障碍性疾病药物筛选模型
- Author:
Li LIU
1
;
Siran PEI
;
Huali WU
;
Qingshun ZHAO
;
Jixian PENG
;
Jing SHANG
Author Information
1. 中国药科大学江苏省中药评价与转化重点实验室
- Publication Type:Journal Article
- Keywords:
zebrafish;
tyrp1a;
pigmentation disorders;
drug screening model
- From:
Journal of China Pharmaceutical University
2016;47(6):740-743
- CountryChina
- Language:Chinese
-
Abstract:
To effectively screen treating pigmentation disorders drug, a new transgenic zebrafish drug screening model was constructed. Green fluorescent protein(GFP)were drived by tyrosinase-related protein 1a (tyrp1a)promoter specific expression in melanocyte. Effect of N-Phenylthiourea(PTU)and alpha-melanaocyte stimulating hormone(α-MSH)on the GFP expression and melanogenic of tyrp1a: eGFP zebrafish were photographed under the steromicroscope. Results showed that PTU could significantly inhibit the melanogenic and expression of GFP of zebrafish. As compared with control group, α-MSH treatment resulted in marked stimulation of body pigmentation and expression of GFP. In conclusion, a new transgenic zebrafish drug screening model was successfully established, which can be used for treating pigmentation disorders.
