Chemical constituents from the leaves of Anisopus mannii and the melanogenesis inhibitory activities

VernacularTitle:Anisopus mannii 叶的化学成分和抑制黑色素生成活性研究
Author: Feng YE ¹ ; Qinglin LYU ; Wanfang ZHU ; Feng FENG ; Jie ZHANG
Author Information

1. 中国药科大学中药学院
Publication Type:Journal Article
Keywords: Anisopus mannii; chemical constituents; structural identification; melanogenesis-inhibitory activity
From: Journal of China Pharmaceutical University 2018;49(6):676-681
CountryChina
Language:Chinese
Abstract: The phytochemical studies on the leaves of Anisopus mannii led to the isolation of seven compounds by silica gel, ODS, DIAION HP-20, Sephadex LH-20 colunmn chromatographer, their structures were elucidated on the basis of the spectroscopic analyses(NMR, HRMS)and the comparisons with the literatures as 3β-acetoxylup-20(29)-ene(1), 1-acetoxy-2-isopropyl-1-tridecene(2), rutin(3), 3, 6′-diferuloylsucrose(4), 3-O-β-D-glucopyranosyl-3-β-hydroxyolean-12-en-28-oic acid 28-O-［α-L-rhamnopyra- nosyl-(1→2)-β-D-glucopyranosyl］ ester(5), conduritol A(6), hoyacarnoside I(7). Meanwhile, the isolated compounds were evaluated for their inhibitory activities against melanogensis in B16 melanoma cells, as the results, all compounds exhibited melanogenesis-inhibitory activity and compound 5 showed a strongest activity(Melanin content: (27. 4±3. 5)%, Cell Viability: (54. 9±5. 6)% with a concentration of 30 μmol/L)which could be further developed.