Effects of bone marrow mesenchymal stem cell transplantation on the expression of stromal cell-derived factor-1α and vascular endothelial growth factor in rats with acute hepatic failure
10.3760/cma.j.issn.1007-3418.2018.10.004
- VernacularTitle: 骨髓间充质干细胞移植对急性肝衰竭大鼠基质细胞衍化生长因子-1α、血管内皮生长因子表达的影响
- Author:
Shufang YUAN
1
;
Zhiying LIU
2
;
Xiamuguli AYI
1
;
Rongjiong ZHENG
3
;
Yuexin ZHANG
3
Author Information
1. Department of Infectious Diseases, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
2. Department of Pathology, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
3. Department of Infectious Diseases, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
- Publication Type:Journal Article
- Keywords:
Liver failure, acute;
Stem cells;
Stromal cell-derived growth factor-1α
;
Vascular endothelial growth factor
- From:
Chinese Journal of Hepatology
2018;26(10):750-755
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the curative effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the expression of stromal cell-derived growth factor (SDF-1 α) and vascular endothelial growth factor (VEGF) in rats with acute hepatic failure, and to compare the effects of two transplantation pathways.
Methods:Eighty-four rats with acute liver failure (ALF) induced by D-galactosamine combined with lipopolysaccharide were randomly divided into control group, tail vein and portal vein transplantation group. The latter two groups were injected allogenic BMSCs into the tail vein and portal vein. Blood samples and liver tissue samples were collected at 24, 72, 120, and 168h after transplantation to detect serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The improvement of liver function before and after BMSCs transplantation was compared. The expression of VEGF and SDF-1a in liver tissue was detected by immunofluorescence and Western blot. Data measurement between two groups was performed by analysis of variance and the correlation analysis was performed by Spearman’s rank correlation coefficient.
Results:Serum ALT and AST levels in the tail vein and portal vein transplantation group peaked at 24 h after transplantation, which were (134.60 ± 58.08 IU/L), (179.20 ± 86.68 IU/L), and (131.00 ± 54.47 IU/L), (173.50 ± 93.10 IU/L). In addition, 168h after transplantation it decreased to (46.10 ± 8.40 IU/L), (95.67 ± 13.80 IU/L) and (19.30 ± 1.30 IU/L), (54.30 ± 6.00 IU/L). After 120 and 168 hours of BMSCs transplantation, the levels of serum ALT and AST in tail vein and portal vein transplantation group were significantly higher than control group (F ≥ 12.51, P < 0.01). The results of western blot and immunofluorescence showed that the expression levels of SDF-1α and VEGF protein in the two BMSCs transplantation groups increased with the improvement of liver function, and the difference was statistically significant at 120 and 168 hours after transplantation (F ≥ 9.069, P < 0.05). There was no significant difference in the expression of SDF-1a and VEGF between the tail vein and portal vein transplantation groups (P > 0.05). Correlation analysis showed that the expression levels of SDF-1α and VEGF in liver tissues were positively correlated (r = 0.923, P < 0.05).
Conclusion:BMSCs transplantation can promote the secretion of VEGF for recovery of liver function to reduce the degree of inflammation and necrosis in rats with ALF.
