HSP90 inhibitor 17-AAG plays an important role in JAK3/STAT5 signaling pathways in HTLV-1 infection cell line HUT-102
10.3760/cma.j.issn.0253-2727.2017.08.012
- VernacularTitle: 热休克蛋白90抑制剂17-AAG对HUT-102细胞株JAK3/STAT5信号通路的影响
- Author:
Qingqing YANG
1
;
Huo TAN
;
Zhiping FU
;
Qiang MA
;
Jinlong SONG
Author Information
1. The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China
- Publication Type:Journal Article
- Keywords:
Leukemia, T cell;
HSP90 heat-shock proteins;
Inhibitor
- From:
Chinese Journal of Hematology
2017;38(8):710-715
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze whether heat-shock protein 90 (HSP90) be involved in a permanently abnormal activated JAK/STAT signaling in ATL cells in vitro.
Methods:The effect of 17-AAG on proliferation of ATL cell lines HUT-102 was assessed using CCK8 at different time points. Cell apoptosis was measured by flow cytometry. The specific proteins HSP90, STAT5, p-STAT5 and JAK3 were detected by Western blotting.
Results:Overexpression of HSP90 in HUT-102 cell lines was disclosed (P<0.05) , and constitutive activation of JAK3/STAT5 signaling was observed in HTLV-1-infected T-cell lines but not in normal PBMCs; Treatment of ATL cell lines with 17-AAG led to reduced cell proliferation, but there was no significant change in terms of cell proliferation when the concentration of 17-AAG between 2 000-8 000 nmol/L (P>0.05) . 17-AAG induced cell apoptosis in different time-points and concentrations. 17-AAG don’t affect the expression of JAK3 gene.
Conclusion:This study indicated that JAK3 as HSP90 client protein was aberrantly activated in HTLV-1-infected T-cell lines, leading to constitutive activation of p-STAT5 in JAK/STAT signal pathway, which demonstrated that HSP90-inhibitors 17-AAG inhibited the growth of HTLV-1-infected T-cell lines by reducing cell proliferation and inducing cell apoptosis.
