Clinical value of p16/Ki-67 immunocytochemical dual staining in cervical cancer screening
10.3760/cma.j.issn.0253-3766.2017.08.015
- VernacularTitle: p16/Ki－67免疫细胞化学双染在宫颈癌筛查中的应用价值
- Author:
Hairui WANG
1
;
Guangdong LIAO
2
;
Wen CHEN
3
;
Youlin QIAO
3
;
Yu JIANG
1
Author Information
1. School of Public Health, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
2. Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China
3. Department of Epidemiology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
- Publication Type:Journal Article
- Keywords:
p16/Ki-67;
Cervical intraepithelial neoplasia;
Human papillomavirus;
Cervical cancer screening
- From:
Chinese Journal of Oncology
2017;39(8):636-640
- CountryChina
- Language:Chinese
-
Abstract:
Objective:to investigate the clinical value of p16/Ki-67 immunocytochemical dual staining (abbreviated as p16/Ki-67 dual staining) in cervical intraepithelial neoplasia (CIN) and cervical cancer screening.
Methods:From July to November 2015, a total of 980 women attending cervical cancer screening and receiving high-risk human papillomavirus (HR-HPV) test and thinprep cytologic test (TCT) were included in the study. p16/Ki-67 immunocytochemical dual staining was performed on residual cytologic specimens and compared with histopathology results.
Results:The expression risks of p16/Ki-67 in HPV16/18 group and another HR-HPV group were higher than HPV negative group, with an odds ratio of 10.64 (95%CI: 5.66~20.02) and 5.40 (95%CI: 3.62~8.04), respectively. The positive rate of p16/Ki-67 increased with the grade of TCT and histologic diagnosis, and was higher in both CIN2 and CIN3 groups than normal group (P<0.05). The sensitivity of p16/Ki-67 to detect CIN2+ and CIN3+ lesions was 89.3% and 94.1%, respectively, and the specificity was 69.3% and 66.8%, respectively. The sensitivity of TCT to detect CIN2+ and CIN3+ lesions was 60.7% and 64.7%, respectively, and the specificity was 49.3% and 49.1%, respectively.
Conclusions:Compared with TCT, p16/Ki-67 dual staining has higher sensitivity and specificity. It can identify high-grade cervical lesions and guide the classification of CIN. p16/Ki-67 dual staining in conjunction with HPV test may be considered as an efficient method for cervical cancer screening.
