Effect of polydimethylsiloxane matrix elasticity on osteogenic differentiation of rat marrow stromal cells
10.3760/cma.j.issn.1002-0098.2017.08.009
- VernacularTitle: 聚二甲基硅氧烷基底弹性对大鼠骨髓间充质干细胞骨向诱导分化的影响
- Author:
Yue MU
1
;
Jizhi ZHAO
1
;
Chun YANG
2
;
Yan ZU
2
;
Qian LI
1
Author Information
1. Department of Stomatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China
2. Institute of Biomechanics and Medical Engineering, School of Aerospace, Tsinghua University, Beijing 100083, China
- Publication Type:Journal Article
- Keywords:
Bone Mesenchymal stem cells;
Polydimethylsiloxane;
Matrix elasticity;
Osteogenic differentiation
- From:
Chinese Journal of Stomatology
2017;52(8):492-498
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of polydimethylsiloxane (PDMS) matrix elasticity on osteogenic differentiation of rat marrow stromal cells (rBMSC).
Methods:A series of PDMS composite substrates with different elastic modulus were constructed by adjusting the relative concentrations of cross-linking agent. The Young's modulus was used to describe the elasticity of PDMS after measurement by atomic force microscope (AFM). After surface modification, rBMSC was seeded on PDMS matrix, and 7 days after rBMSC was cultured on the five different Young's moduli matrix, the differences of osteogenic differentiation of rBMSC were observed by the method of real-time PCR, Western blotting, and alkaline phosphatase assay.
Results:The PDMS was suitable for cell culture after surface modification, and by altering the concentration of cross-linking agent, PDMS could mimic the majority of the tissues' elasticity in vivo. The related osteogenic differentiation markers expression showed significant difference between the five matrixes (P<0.05), including type Ⅰ collagen (Col-Ⅰ), osteocalcin (OCN), osteopontin (OPN) and bone morphogenetic protein 2 (BMP2). The expression of osteogenic markers was up-regulated in the group that the Young's modulus was (354.1±40.9) kPa (P<0.05).
Conclusions:PDMS is a tunable elasticity matrix which could be used in the investigation of inducing rBMSCs into osteoblastic lineages. PDMS substrate stiffness has an obvious influence on rBMSC osteogenic differentiation.
