Application of p16/Ki-67 immunocytochemistry in triage of patients with atypical squamous cells of undetermined significance
10.3760/cma.j.issn.0529-5807.2017.07.007
- VernacularTitle: p16/Ki-67双染检测在意义不明的不典型鳞状细胞分流诊断中的应用
- Author:
Rongrong JIN
1
;
Hongwei MA
;
Tianyu CHEN
;
Rong RONG
;
Yan WU
;
Shilan LI
;
Zhihong ZHANG
Author Information
1. Department of Pathology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
- Publication Type:Journal Article
- Keywords:
Cervical intraepithelial neoplasia;
Cyclin-dependent kinase inhibitor p16;
Ki-67 antigen;
Human papillomavirus 16;
Cytological techniques
- From:
Chinese Journal of Pathology
2017;46(7):481-484
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the clinical value of p16/Ki-67 immunocytochemistry in patients with atypical squamous cells of undetermined significance(ASC-US).
Methods:One hundred and seventy-one cases of thin-prep cytology test (TCT) diagnosed as ASC-US underwent p16/Ki-67 immunocytochemistry. All patients had colposcopy and biopsy from March 2015 to January 2016. Ninety of the 171 cases underwent high-risk HPV test at the same time.
Results:p16/Ki-67 immunocytochemistry was positive in 43.9% (75/171) of the 171 cytology samples; the sensitivity and specificity of p16/Ki-67 immunocytochemistry were 77.6%(52/67) and 77.9%(81/104) in detecting CIN2+ , and the positive and negative predictive value were 69.3%(52/75) and 84.4%(81/96), respectively. The sensitivity and specificity in diagnosing CIN2+ were 100.0%(34/34) and 10.7%(6/56) for HPV test, and the positive and negative predictive value were 40.5%(34/84) and 6/6. p16/Ki-67 immunocytochemistry showed lower sensitivity but obviously higher specificity than high-risk HPV detection.
Conclusion:p16/Ki-67 immunocytochemistry is a good triage test for identifying CIN2+ in ASC-US specimens.
