A Study on the establishment of immune thrombocytopenia model induced by anti-platelet GPⅠbα antibodies
10.3760/cma.j.issn.0253-2727.2017.05.007
- VernacularTitle: 抗血小板GPⅠbα抗体诱导建立原发免疫性血小板减少症模型的研究
- Author:
Kangxi ZHOU
1
;
Rong YAN
;
Mengxing CHEN
;
Jun LIU
;
Qingya CUI
;
Renping HU
;
Yancai LIU
;
Yang ZHANG
;
Changgeng RUAN
;
Kesheng DAI
Author Information
1. Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University; Key Laboratory of Thrombosis and Hemostasis, Ministry of Health; Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215006, China
- Publication Type:Journal Article
- Keywords:
Thrombocytopenia;
Models, animal;
Platelet Membrane GlycoproteinⅠb
- From:
Chinese Journal of Hematology
2017;38(5):390-393
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish primary immune thrombocytopenia (ITP) animal model induced by anti-platelet membrane glycoprotein GPⅠbα antibodies AN51 and R300.
Methods:Twenty guinea pigs (6-8 week) were divided into 4 groups. Five guinea pigs in each group were intravenously injected with different doses of AN51 (0.05, 0.1, 0.2 μg/g) and 0.2 μg/g IgG as control. The whole blood was collected from inner angular venous plexus. Platelets number was determined by an automated cell counter and Swiss-Jim method. Then, the similar protocol was used to establish ITP nude mice model by intraperitoneal injection of different concentrations of anti-platelet GPⅠbα antibody R300, respectively.
Results:①Five minutes after intravenous injection of AN51 at 0.05, 0.1 and 0.2 μg/g, the platelet counts of guinea pigs reduced about 0-5%, 50%-60% and 70%-80% compared to the control group, respectively. The difference was statistically significant (P<0.01) . ②Six hours after intraperitoneal injection of R300 at 0.05, 0.1, 0.2 μg/g, the platelet counts of nude mice decreased about 20%-30%, 60%-70% and 80%-90% compared to the control group, respectively. The difference was statistically significant (P<0.01) . The nude mice, injected 0.2 μg/g R300 once a day for 2 weeks, showed typical ITP clinical manifestations including large number of petechiaes or ecchymoses on limbs, head and abdomen.
Conclusion:AN51 at 0.2 μg/g and R300 at 0.2 μg/g could establish stable ITP model in guinea pigs and nude mice respectively.
