Influence of sodium nitrite exposure on sulfhemoglobin and hydroxyl radicals in mice
10.3760/cma.j.issn.1001-9391.2017.05.013
- VernacularTitle: 亚硝酸钠对小鼠体内硫血红蛋白和羟自由基的影响
- Author:
Zhaoqiang ZHANG
1
;
Li LIN
;
Chunzhi ZHANG
Author Information
1. Key Laboratory of Occupational Health & Environmental Medicine, School of Public Health, Jining Medical University, Jining 272067, China
- Publication Type:Case Reports
- Keywords:
Sodium nitrite;
Sulfhemoglobin;
Hydroxyl radical
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(5):366-368
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of sodium nitrite exposure on sulfhemoglobin and hydroxyl radicals in mice.
Methods:A total of 60 mice were randomly divided into low-, middle-, and high-dose groups (the concentrations of sodium nitrite were 0.055 mg/ml, 0.110 mg/ml, and 0.220 mg/ml, respectively) and control group (treated with distilled water) , with 15 mice in each group (male/female ratio=1: 1) . A free-drink model was applied and the duration of exposure was 2 weeks. The body weight of all mice was recorded before exposure and at weeks 1 and 2 of exposure. At the end of exposure, the mice were treated with intraperitoneally injected sodium salicylate to capture the hydroxyl radicals and produce 2, 5-dihydroxybenzoic acid and 2, 3-dihydroxybenzoic acid, and high-performance liquid chromatography was used to measure their content. Spectrophotometry was used to measure the relative content of sulfhemoglobin.
Results:At week 2 of exposure, the low-, middle-, and high-dose groups had significantly lower body weight than the control group (22.8±2.8 g/21.6±2.8 g/21.2±3.0 g vs 25.6±2.2 g, P<0.05) . The low-, middle-, and high-dose groups had a significantly higher total content of hydroxyl radicals than the control group[ (0.015 3±0.006 5) μg/ml, (0.016 4±0.017 2) μg/ml, and (0.062 7±0.091 0) μg/ml vs (0.009 ±0.007 3) μg/ml, P<0.05]. The relative content of sulfhemoglobin was 1.54%±0.73%, 2.22%±0.44%, and 2.80%±0.69%, respectively, in the low-, middle-, and high-dose groups, and the middle- and high-dose groups had a significant increase in the relative content of sulfhemoglobin compared with the control group (2.22%±0.44%/2.80%±0.69% vs 1.76%±0.60%, P<0.05) . The content of hydroxyl radicals was positively correlated with the relative content of sulfhemoglobin (r=0.837, P<0.05) .
Conclusion:Sodium nitrite exposure can increase the content of sulfhemoglobin and hydroxyl radicals in blood, and there is a positive correlation between them.
