Value of DNA content in comet tail in predicting changes in blood cell counts in workers exposed to benzene
10.3760/cma.j.issn.1001-9391.2017.03.003
- VernacularTitle: 彗星尾部DNA含量对苯接触工人血细胞数量变化的预测
- Author:
Boxuan LIANG
1
;
Xuexing LIU
;
Liping CHEN
;
Aichu YANG
;
Ming HUANG
;
Qifei DENG
;
Wen CHEN
;
Yongmei XIAO
Author Information
1. School of Public Health, Sun Yat-sen University, Guangzhou Key Laboratory of Environmental Pollution and Health Risk Assessment, Guangzhou 510080, China
- Publication Type:Case Reports
- Keywords:
Benzene;
Occupational exposure;
TailDNA;
Blood cell count
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(3):171-175
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the value of DNA content in comet tail (TailDNA) in predicting the changes in peripheral blood cell counts in workers exposed to benzene.
Methods:In 2011, cluster sampling was used to select 150 male workers exposed to benzene in a petrochemical factory. Cubital venous blood and urine samples were collected for routine blood rest, comet assay, and measurement of s-phenylmercapturic acid (SPMA) and urine creatinine. The population was divided into groups according to urinary SPMA or TailDNA, and routine blood test results within 3 years were collected to analyze the changes in blood cell counts.
Results:The low-SPMA group had significantly higher white blood cell and neutrophilcounts in all years than the high-SPMA group (P<0.05) . The low-Tail DNA group had a significant increase in platelet count from 2012 to 2014 (P<0.05) , while the high-Tail DNA group had no significant change (P>0.05) . During the 4-year period, the high-TailDNA group had a significantly lower red blood cell count than the low-TailDNA group (P<0.05) . The high-TailDNA group showed a gradual reduction in white blood cell count over time (β=-0.113, P<0.05) , and the low-TailDNA group showed no trend of the reduction in white blood cell count (P>0.05) .
Conclusion:TailDNA can be used to predict the changes in blood cell counts in workers exposed to benzene.
