The effect of complement C3a receptor antagonist in the kidney immune injury in trichloroethylene-sensitized mice
10.3760/cma.j.issn.1001-9391.2017.03.001
- VernacularTitle: 补体C3a受体拮抗剂对三氯乙烯致敏小鼠肾脏免疫性损伤的影响
- Author:
Peng YANG
1
;
Dandan ZANG
;
Xiaodong YANG
;
Bodong LI
;
Xian WANG
;
Jiaxiang ZHANG
;
Qixing ZHU
Author Information
1. Department of Occupational and Environment Health, School of Public Health, Anhui Medical University, Hefei 230032, China
- Publication Type:Journal Article
- Keywords:
Trichloroethylene;
Kidney;
C3aR;
Interleukins;
Tumor necrosis factor
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(3):161-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of complement C3 a-C3a receptor in the kidney immune inju-ry in trichloroethylene-sensitized mice by using C3a receptor specific antagonist C3aRA and discuss the patho-genesis of kidney injury in occupational dermatitis medicamentosa-like of trichloroethylene (ODMLT) .
Methods:42 female 6~8 weeks old BALB/c mice of specific pathogen free were randomly divided into blank control group (5) , solvent control group (5) , TCE treatment group (16) and TCE+C3aRA treatment group (16) . The TCE treat-ment group and TCE+C3aRA treatment group were further divided into the sensitized group and the non-sensi-tized group according to the skin sensitization test score. Renal function was detected by biochemical detection kit; expression of C3aR in kidney tissue was detected by qPCR; expression of IL-1β and TNF-α protein were de-tected by immunohistochemical.
Results:Compared with solvent control group and corresponding non-sensitized group, CRE and BUN in TCE sensitized group and TCE + C3aRA sensitized group were significantly increased (P<0.05) . Compared with TCE sensitized group, CRE and BUN in TCE+C3aRA sensitized group were signifi-cantly decreased (P<0.05) . Compared with solvent control group and TCE non-sensitized group, the expression level of C3aR gene in kidney tissue in TCE sensitized group was significantly increased (P<0.05) . There was a large number of IL-1β and TNF-α protein expression in kidney tissue in TCE sensitized group and TCE+C3aRA sensitized group. Compared with the TCE sensitized group, the expression level of IL-1β and TNF-α protein in kidney tissue in TCE+C3aRA sensitized group was significantly decreased (P<0.05) .
Conclusion:C3a-C3aR may be involved in the kidney immune injury in TCE sensitized mice, C3aRA has a protective effect on the kid-ney immune injury in TCE sensitized mice.
