Effect of triptolide on human oral cancer cell proliferation and PTEN gene mRNA expression in oral cancer
10.3760/cma.j.issn.1002-0098.2017.01.009
- VernacularTitle: 雷公藤甲素对人口腔鳞状癌细胞增殖及PTEN基因表达的影响
- Author:
Leijie PEI
1
;
Jingdong LI
2
;
Zhonghua ZHAO
1
;
Jian LI
3
;
Ruiying LIANG
1
;
Zhiqiang XIN
2
Author Information
1. Department of Prosthodontics, School of Stomatology, North China University of Science and Technology, Tangshan 063000, China
2. Department of Oral and Maxillofacial Surgery, Tangshan Xiehe Hospital, Tangshan 063004, China
3. Department of Stomatology, Tangshan People's Hospital, Tangshan 063000, China
- Publication Type:Journal Article
- Keywords:
Oral sprays;
Tripterygium;
Phosphates and tensin homologue deleted on chromosome ten gene
- From:
Chinese Journal of Stomatology
2017;52(1):44-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of triptolide on human oral cancer cell (HB) proliferation and phosphates and tensin homologue deleted on chromosome ten gene (PTEN) mRNA expression in oral cancer.
Methods:The cancer cells were cultured in the medium containing triptolide of different concentrations for 24, 48 and 72 h. Methyl thiazolyl tetrazolium (MTT) method was used to test the rate of growth inhibition of cancer cells, flow cytometer to detect the change of cell cycle and reveres transcription-PCR (RT-PCR) to examine the expression of PTEN mRNA. The expression of PTEN protein was examined by Western blotting.
Results:The rate of growth inhibition was (26.92 ± 0.14)%, (38.67 ± 0.11)%, (72.62 ± 0.89)% and (90.42 ± 0.28)%, respectively. The corresponding expression of PTEN mRNA was (3.59±0.21)%, (5.27±0.40)%, (7.18±0.44)% and (9.16±0.50)%, respectively and the corresponding A value of PTEN protein was 0.135±0.007, 0.410±0.020, 0.447±0.017 and 0.884±0.066, respectively. The proportion of G1 phase cells increased from (58.78±0.98)% to (84.13±0.47)%, but the proportion of S phase cells decreased from (25.40±0.43)% to (9.41±0.73)%.
Conclusions:The triptolide not only had inhibitory effect on the HB proliferation, but also affected the cell cycle.
