Qualitative and quantitative analyses of Chrysanthemum morifolium ‘Fubaiju’ by LC-MSn and HPLC-UV
10.11665/j.issn.1000-5048.20190509
- VernacularTitle:福白菊化学成分LC-MSn定性分析及HPLC-UV法多成分含量测定
- Author:
Junyan LI
1
;
Thi Anh PHAM
;
Zhongxuan SUN
;
Xuexiang CHEN
;
Wencai YE
;
Hao WANG
Author Information
1. 中国药科大学中药制药系
- Publication Type:Journal Article
- Keywords:
Chrysanthemum morifolium ‘Fubaiju’;
LC-MSn;
HPLC-UV;
qualitative analysis;
content determination
- From:
Journal of China Pharmaceutical University
2019;50(5):565-571
- CountryChina
- Language:Chinese
-
Abstract:
A qualitative analytical method of liquid chromatography coupled with mass spectrometry(LC-MSn)was developed for the identification of main constituents in Chrysanthemum morifolium ‘Fubaiju’. High-performance liquid chromatography(HPLC)was developed for the quantification of five active components, including chlorogenic acid(1), luteolin-7-O-β-D-glucopyranoside(2), luteolin-7-O-β-D-glucopyranuronide(3), 3, 5-Di-caffeoylquinic acid(4), and apigenin-7-O-β-D-glucopyranoside(5). A total of 22 compounds, including 13 flavonoids and 9 phenolic acids, were identified based on their retention behaviors, UV profiles and MS fragment information. Furthermore, a validation method with good linearity(r> 0. 999 9), precision, stability, repeatability and recovery was successfully applied for simultaneous determination of five major components in 10 batches of C. morifolium ‘Fubaiju’ by HPLC-UV method. The established method was proved to be a validation strategy for the quality evaluation of C. morifolium ‘Fubaiju’.
