Experimental study of 99Tcm-3PRGD2 imaging on the angiogenesis of rheumatoid arthritis

Yu WU; Guojian ZHANG; Xuemei WANG; Xin′ai WU; Zhenfeng ZHAO; Kaixiu ZHANG; Ridong JIA

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Experimental study of ⁹⁹Tc^m-3PRGD₂ imaging on the angiogenesis of rheumatoid arthritis

VernacularTitle: ⁹⁹Tc^m－3PRGD₂用于类风湿关节炎血管形成显像的实验研究
Author: Yu WU ¹ ; Guojian ZHANG ² ; Xuemei WANG ² ; Xin′ai WU ² ; Zhenfeng ZHAO ² ; Kaixiu ZHANG ² ; Ridong JIA ²
Author Information

1. Department of Nuclear Medicine, Affiliated People′s Hospital of Inner Mongolia Medical University, Hohhot 010020, China; Department of Nuclear Medicine, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010059, China
2. Department of Nuclear Medicine, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010059, China
Publication Type:Journal Article
Keywords: Arthritis, rheumatoid; Synovial membrane; Neovascularization, pathologic; Radionuclide imaging; Arg-Gly-Asp; Rats
From: Chinese Journal of Nuclear Medicine and Molecular Imaging 2018;38(8):549-553
CountryChina
Language:Chinese
Abstract: Objective:To observe the uptake of ⁹⁹Tc^m-3 polyethylene glycol Arg-Gly-Asp dimer (3PRGD₂) in rat models of rheumatoid arthritis (RA), in order to provide theoretical foundation for early diagnosis of RA.
Methods:The healthy female SD rats were divided into collagen induced arthritis (CIA) group (n=100), osteoarthritis (OA) group (n=20) and control group (n=20). Bovine collagen type Ⅱ emulsion was used for arthritis induction to establish CIA models. OA models were established by injection of L-cysteine and papain. Gamma imaging was performed before and 15 d, 30 d after the model establishment. The mediastinum was selected as non-target (NT) area and the target (T)/NT ratios were calculated. The serum levels of vascular endothelial growth factor (VEGF), tumor necrosis factor-α (TNF-α), α_Vβ₃ before and after the model establishment were measured. Pathological and immunohistochemical detection were performed. One-way analysis of variance, Pearson and Spearman correlation analyses were used to analyze the data.
Results:There were 58 CIA models successfully established. Before the model establishment, T/NT ratios of CIA group, OA group and control group were 0.215±0.049, 0.210±0.050, 0.209±0.051, respectively (F=0.093, P>0.05). The T/NT ratios of the above three groups were 0.405±0.230, 0.223±0.045, 0.211±0.049 (F=12.601, P<0.05) 15 d post-model establishment, and those were 0.572±0.182, 0.238±0.045, 0.212±0.055 (F=65.147, P<0.05) 30 d post-model establishment. T/NT ratios of CIA group were positively correlated with the levels of serum VEGF, TNF-α, α_Vβ₃, pathological score and the levels of immunohistochemical markers (VEGF, TNF-α, α_Vβ₃, CD31, CD34; r values: 0.391-0.721, r_s values: 0.365-0.669, all P<0.05).
Conclusion:⁹⁹Tc^m-3PRGD₂ can be specifically up-taken by RA lesions in rat models, thus has the potential in early synovial neovascularization imaging of RA.