Affinity characterization of GE11 peptide to brain metastases from epidermal growth factor receptor-overexpressing human breast cancer
10.3760/cma.j.issn.2095-2848.2018.08.005
- VernacularTitle: GE11多肽对表皮生长因子受体阳性乳腺癌脑转移瘤的靶向性研究
- Author:
Lin LI
1
;
Fang NIE
1
;
Jian YANG
2
;
Qian XUE
1
;
Jianqiong ZHANG
3
;
Gaojun TENG
1
Author Information
1. Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Medical School of Southeast University, Nanjing 210009, China
2. Department of Physiology, Medical School of Southeast University, Nanjing 210009, China
3. Department of Microbiology and Immunology, Medical School of Southeast University, Nanjing 210009, China
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Neoplasm metastasis;
Brain;
Receptor, epidermal growth factor;
Peptides;
Tumor cells, cultured;
Mice, nude
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2018;38(8):543-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the feasibility of GE11 peptide targeting to brain metastases from epidermal growth factor receptor (EGFR)-positive breast cancer and thereby explore the potential for the construction of specific targeting molecular probe.
Methods:The EGFR expression level of triple negative breast cancer cell line (MDA-MB-231-BR) was detected using Western blot and flow cytometry. GE11 peptide targeting property was tested using immunofluorescence staining and flow cytometry in MDA-MB-231-BR cells and in the subcutaneous tumor model. GK-13 peptide was selected as the control peptide. Two-sample t test was used for data analysis.
Results:Western blot and flow cytometry showed that EGFR expression in MDA-MB-231-BR cells was 88.60%, significantly higher than those in other cells. Flow cytometry results indicated that the cell binding rate of MDA-MB-231-BR with GE11 peptide (10 μmol/L) was significantly higher than that with control peptide (38.4% vs 0.6%; t=16.941, P<0.01). Cell immunofluorescence staining results showed GE11 peptides were detected with stronger signal after being incubated with cells, and the combination was concentration- and temperature-dependent. In vivo experiment results showed that the signal of GE11 peptide was stronger in tumor site than that in other organs or tissues.
Conclusion:GE11 peptide may specifically target the brain metastases of EGFR-positive breast cancer.
