Studies on the DNA damage in the transformed bronchial epithelial cells induced by hexavalent chromium
10.3760/cma.j.issn.1001-9391.2018.07.001
- VernacularTitle: 六价铬致16HBE细胞DNA损伤相关恶性转化的研究
- Author:
Xiaohu REN
1
;
Weixue LU
;
Zhihong CHEN
;
Wei LIU
;
Shuqi WANG
;
Nuanyuan LUO
;
Jianjun LIU
Author Information
1. Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China
- Publication Type:Journal Article
- Keywords:
Hexavalent chromium;
Cell malignant transformation;
DNA damage
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(7):481-484
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate DNA damage in the transformed human bronchial epithelial cells (16HBE) induced by hexavalent chromium (Cr6+) and further elucidate the potential carcinogenesis mechanism of Cr6+.
Methods:16HBE were treated with different concentration of Cr6+ (0, 0.625, 1.25, 2.5 μmol/L) for 15 weeks. The malignant degrees of transformed cells were identified by the assays for anchorage-independent growth and tumorigenicity. According to the single cell gel electrophoresis (SCGE) assay, the DNA damage rate was calculated. The expression level of 53BP1 was determined by Western blot.
Results:Chromium-treated cells could form colonies in soft agar and tumors in nude mice. Compared with the control group, colony formation efficiency of 1.25μmol/L and 2.5 μmol/L Cr6+-treated cells in soft agar showed significant increases (p<0.05) . The 2.5 μmol/L Cr6+-treated cells also formed tumors subcutaneously in nude mice. Cr6+ could cause different degree of DNA damage to 16HBE cells in a dose-dependent manner. In addition, Western blot analyses showed that 53BP1 was aberrantly down-regulated at 2.5 μmol/L dose and has no significant changes at 0.625 μmol/L and 1.25 μmol/L dose under the treatment of Cr6+.
Conclusion:The declined expression of 53BP1 may mediate Cr6+-induced DNA damage and further involved in the cell malignant transformation.
