Applications of the Fe3O4 nanocomposite modified by Humic Acid in determination of S-phenylmercapturic acid in urine by dispersive solid-phase extraction and HPLC
10.3760/cma.j.issn.1001-9391.2018.06.021
- VernacularTitle: 腐植酸改性磁性纳米材料分散固相萃取-高效液相色谱法在尿苯巯基尿酸检测中的应用
- Author:
Haipeng YE
1
;
Yanpeng SHI
;
Ji SHAO
;
Siwei TAN
;
Xiaoyue SHAN
;
Lei ZHANG
;
Chengjian CAO
Author Information
1. Hangzhou Occupational Disease Prevention and Control Hospital, Hangzhou 310014, China
- Publication Type:Journal Article
- Keywords:
S-phenylmercapturic Acid;
Uurine;
Magentic Dispersive Solid-phase Extraction;
High Performance Liquid Chromatography
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2018;36(6):463-465
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for determination the S-phenylmercapturic acid in urine by dispersive solid-phase extraction using Humic Acid/Fe3O4 magnetic nanocomposite as adsorbent.
Methods:The 5 ml of urine samples were adjusted to pH 1.0 and extracted by Fe3O4@HA. Then the analytes were separated on EC-C18 capillary column and detected by HPLC-VWD. The S-phenylmercapturic acid was characterized by the retention time and quantified by peak area and external standard method.
Results:The standard curves of SPMA showed significant linearity between 0.04~1.00 mg/L (r=0.999 7) . The average recovery was 94.2%~102.4%. The intra-day and inter-day precisions (RSD) were 2.9~6.7% (n=6) and 3.1~7.5% (n=6) respectively. The detect limit of SPMA was 0.012 g/L (S/N=3) .
Conclusion:This method is simple, rapid, sensitive and accurate. It is applicable for determination of SPMA in the urine of works who were exposed to benzene.
