Genotype I of Japanese encephalitis virus as the dominant genotype among mosquitoes in Shandong
- VernacularTitle: 山东省蚊虫中以基因1型乙型脑炎病毒流行为主
- Author:
Weijia ZHANG
1
;
Xiaojuan LIN
2
;
Aiguo LIU
3
;
Shihong FU
4
;
Guifang LIU
2
;
Nan SHAO
4
;
Qianying WANG
4
;
Zexin TAO
2
;
Ying HE
4
;
Wenwen LEI
4
;
Guodong LIANG
4
;
Aiqiang XU
2
;
Li ZHAO
1
;
Huanyu WANG
4
Author Information
- Publication Type:Journal Article
- Keywords: Japanese encephalitis virus; Phylogenetic analysis; Virus isolation
- From: Chinese Journal of Experimental and Clinical Virology 2018;32(1):53-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analysis the genotype of Japanese encephalitis virus (JEV) in mosquitoes from Shandong province.
Methods:Mosquitoes were collected between August and September in Weishan county, Junan county, and Kenli county of Shandong province in 2016. Viruses were isolated by BHK-21 cell and identified by molecular method . Real-Time RT-PCR was conducted to detect the Japanese encephalitis virus carried by the mosquitoes.
Results:A total of 8418 mosquitoes divided into 81 pools including 3 species,Culex tritaeniorhynchus, Anopheles sinensis andArmigeres obturbans . Eight Japanese encephalitis viruses were isolated; 23 pools were positive by JEV specific real-time RT-PCR. Phylogenetic analysis on E sequence of JEV showed all JEV strains belonged to genotype Ⅰ JEV, and new strains that were homogenous with previous JEV strains isolated from Shandong.
Conclusions:Genotype Ⅰ JEV was the dominant genotype in Shandong province.
