Si-PTN inhibits the growth of hypertrophic scar fibroblasts
10.3760/cma.j.issn.1009-4598.2019.06.016
- VernacularTitle: Si-PTN抑制瘢痕成纤维细胞生长
- Author:
Qian ZHANG
1
;
Jie SHI
;
Yaguang TIAN
;
Xiaodian LI
;
Kai TAO
Author Information
1. Department of Reconstructive and Plastic Surgery, The General Hospital of North Military Region, Shenyang 110840, China
- Publication Type:Journal Article
- Keywords:
Hypertrophic scar;
Proliferation;
Apoptosis
- From:
Chinese Journal of Plastic Surgery
2019;35(6):601-606
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the effects of PTN in the growth of hypertrophic scar fibroblasts.
Methods:The primary scar fibroblasts were extracted from fresh hypertrophic scar tissues of 18 patients, to restrict the si-PTN cell line. After successful construction, the scar fibroblasts in logarithmic growth were divided into si-PTN group and control group, transfected with 2 μg si-PTN and control nonsense chains, respectively. MTT, PI staining and Annexin V-FITC/PI assay were used to detect the proliferation and apoptosis of si-PTN and control groups. Western blot and RT-PCR were used to detect the expression level of Cyclin D1, Bcl-2 and Bax in si-PTN and control groups.
Results:The inhibition rates of si-PTN on the proliferation of scar fibroblasts were 14.49%, 13.24%, 20.78% and 23.12% at 1, 2, 3 and 4 days, respectively. The difference was statistically significant compared with the control group (P<0.05). The results of cell cycle experiments showed that the proportion of G0-G1 phase in the si-PTN group was (60.79±3.34)%, which was higher than that of the control group (50.54±1.80)% (P=0.02). The proportion of S phase cells in the si-PTN group was (29.02±2.07)%, which was lower than that in the control group (40.08±2.89)% (P=0.03). The results of apoptosis assay showed that the proportion of early and late apoptotic cells in si-PTN group were (8.17±0.57)% and (6.80±0.74)%, respectively, which were higher than that of the control group (4.35±0.46)%, and (4.70±0.48)% (P<0.05). Western Blot and RT-PCR results showed that compared with the control group, the si-PTN group was down-regulated by Cyclin D1 (43.76%) and Bcl-2 (43.56%), Bax was up-regulated (31.97%) (P<0.05).
Conclusions:si-PTN inhibited the growth of scar fibroblasts.
