Changes of endogenous small interfering RNA in H1-HeLa cells induced by human rhinovirus 16 infection
10.3760/cma.j.issn.1003-9279.2019.03.005
- VernacularTitle: 16型鼻病毒感染引起H1-HeLa细胞的内源性siRNA变化
- Author:
Juan SONG
1
;
Mi LIU
;
Hengshun CHENG
;
Bingtian SHI
;
Wenjun WANG
;
Zhiqiang XIA
;
Qinqin SONG
;
Tianyu CAO
;
Dong XIA
;
Jun HAN
Author Information
1. State Key Laboratory of Infectious Disease Prevention and Control; National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Tereatment of Infections Disdese, Beijing 100052, China
- Publication Type:Journal Article
- Keywords:
siRNA;
Human rhinovirus 16;
High-throughput sequencing
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(3):248-252
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the changes of endogenous small interfering RNA (siRNA) in H1-Hela cells infected with human rhinovirus 16 (HRV 16).
Methods:To determine whether HRV16 infection induced the changes of siRNA, H1-HeLa cells were infected with HRV16 for 12 h, 24 h and 36 h, siRNAs were detected by high-throughput sequencing, second-generation sequencing) and qRT-PCR.
Results:The result showed that siRNA was generated differently at different time points post-infection, among which novel_sir907 and novel_sir1950 decreased at three time points. Further validation by qRT-PCR showed that novel_sir907 decreased at 12 h, 24 h and 36 h post-infection compared with the cell control, but novel_sir1950 increased at 12 h then decreased at 24 h and 36 h.
Conclusions:HRV16 infection induces changes endogenous siRNAs.
