Changes in mass spectrometry of proteins in patients with 1-bromopropane poisoning after treatment
10.3760/cma.j.issn.1001-9391.2019.01.007
- VernacularTitle: 1-溴丙烷中毒患者治疗前后蛋白质谱的变化
- Author:
Rongming MIAO
1
;
Bangmei DING
2
;
Xintian YU
3
;
Baoli ZHU
2
Author Information
1. Wuxi Rehabilitation Hospital, Wuxi 214011, China
2. Institute of Occupational Disease Prevention, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing 210009, China
3. Baoan Shajing Preventive Health Center, Shenzhen 518100, China
- Publication Type:Case Reports
- Keywords:
1-Bromopropane;
Poisoning;
Occupational diseases;
Proteome
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2019;37(1):34-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes in mass spectrometry of proteins in patients with 1-bromopropane (1-BP) poisoning after treatment and their biological functions.
Methods:From May 2016 to December 2017, 3 male patients aged 31-47 years with 1-BP poisoning in Bao'an District of Shenzhen, China were enrolled in this study. The whole blood sample (2 ml) was collected before and after treatment. Label-free mass spectrometry-based proteomics was used for protein identification and quantification. The differentially expressed proteins after treatment were analyzed. Bioinformatics tools were used to analyze the functions of the identified proteins and the biological processes they were involved in.
Results:Proteomic analysis showed that there were 47 proteins that were differentially expressed more than 2-fold (P<0.05) after treatment in the patients with 1-BP poisoning; of them, 27 were up-regulated and 20 were down-regulated in the serum of treated patients. The identified proteins were mainly involved in proteolysis, protein modification, immune response, complement activation, lipoprotein metabolism, signal transduction, and coagulation.
Conclusion:The differentially expressed proteins after treatment can help with the diagnosis, treatment, and prognosis monitoring of 1-BP poisoning and provide potential therapeutic and prognostic markers for 1-BP poisoning treatment.
