Differential proteomic analysis on osteoblasts stimulated by mechanical strain
- VernacularTitle:机械牵张应力刺激成骨细胞的差异蛋白质组学研究
- Author:
Fei fei LI
1
;
Yin DING
1
;
Xue FENG
1
;
Huan WANG
1
;
Fu lin CHEN
2
;
Li wen LI
2
Author Information
1. Department of Orthodontics， School of Stomatology, the Fourth Military Medical University
2. Lab of Tissue Engineering, College of Life Sciences， Northwest University
- Publication Type:Journal Article
- Keywords:
Mechanical strain;
Osteoblastic cells;
Proteomics;
Cell proliferation;
Signal transduction
- From:
Journal of Medical Biomechanics
2010;25(6):E406-E411
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the differentially expressed proteins and clarify the major proteins involved in the molecular mechanism of osteoblasts under mechanical strain loading. Method Saos 2 osteoblastic cells were subjected to 12% elongation for 24 hours by using Flexcell strain loading system. Proteins extracted from Saos 2 cells were separated by two dimensional electrophoresis (2 DE). Differential expressed protein spots among groups were submitted to matrix assisted laser desorption/ionization time of flight mass spectrometer (MALDI TOF MS) assay and peptide mass fingerprinting (PMF) identification. The Swiss Prot and NCBI databases were used to obtain further information about proteins identified. Results Saos 2 stimulated by mechanical strain showed a significant difference in 2 DE system compared with the control group. A total of (1031±41) or (928±25) protein spots were resolved by 2 DE of controls or experimental groups extractions respectively. 17 significant up regulated proteins were identified. These associated proteins fell into 6 groups, including stress reaction, energy metabolism, cell proliferation, reconstruction of cytoskeleton, signaling and osteogenesis. Conclusions The Saos 2 can express differential proteins stimulated by mechanical strains and these proteins may play an important role in molecular mechanism of osteoblasts under mechanical strain loading.
