Effect of Hei Xiaoyaosan on Expressions of Aβ1-42, GSK-3β, NEP, IDE in Hippocampus Area of Alzheimer's Dementia Mice
10.13422/j.cnki.syfjx.20190502
- VernacularTitle: 黑逍遥散对AD模型小鼠海马区Aβ1-42,GSK-3β,NEP,IDE表达的影响
- Author:
Hong-yan WU
1
;
Chun-lin MA
2
;
Shu-mei CUI
2
;
Kai-min ZHU
2
;
Jia-nan LIU
3
;
Qing-tao ZENG
4
Author Information
1. Shenzhen Luohu People's Hospital, Shenzhen 518020, China
2. Gansu University of Chinese Medicine, Lanzhou 730000, China
3. Dingxi Second People's Hospital, Dingxi 743000, China
4. Shenzhen Luohu Hospital of Traditional Chinese Medicine, Shenzhen 518001, China
- Publication Type:Research Article
- Keywords:
Alzheimer's disease;
Hei Xiaoyaosan;
β-amyloid 1-42 peptide(Aβ1-42);
glycogen synthase kinase-3β(GSK-3β);
neprilysin(NEP);
insulin-degrading enzyme(IDE)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(5):36-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of Hei Xiaoyaosan on expressions of β-amyloid 1-42 peptide(Aβ1-42),glycogen synthase kinase-3β(GSK-3β),neprilysin(NEP),insulin-degrading enzyme(IDE) in the hippocampus area of Alzheimer's dementia mice. Method: After weighing, 42 APP/PSI bivalent transgenic mice were randomly divided into 4 groups:10 mice in the model group, 10 mice in the positive drug control group, 11 mice in the high-dose Hei Xiaoyaosan group, and 11 mice in the low-dose Hei Xiaoyaosan group; 10 wild C57BL/6J mice of the same age and strain were used for negative control group. Drugs were administered to mice by gavage once a day for 12 weeks. Then the behavior of all the mice were detected by Morris water maze, the morphological changes in hippocampal neurons were observed by hematoxylineosin(HE) staining, the expressions of Aβ1-42, GSK-3β, NEP and IDE proteins in hippocampus were detected by immunohistochemistry. Result: After 3 months of treatment, compared with negative control groups, the average escaping latency periods prolonged significantly, and the number of cross-platform was decreased significantly in model group (P<0.01), the expressions of Aβ1-42 and GSK-3β proteins in model mice hippocampus were significantly increased (P<0.01), the expressions of NEP and IDE proteins in model mice hippocampus were significantly decreased (P<0.01), suggesting serious damage of hippocampal nerve cells in model group mice according to HE staining; compared with the model group, the escape latency of drug groups were significantly shortened, and the number of crossing platforms was significantly increased (P<0.05), the expressions of Aβ1-42 and GSK-3β proteins in the hippocampus of drug groups were significantly decreased (P<0.01), the expressions of NEP and IDE proteins in the hippocampus of drug groups were significantly increased (P<0.05,P<0.01), suggesting the alleviation in the damage of hippocampal nerve cells in drug groups. Conclusion: Hei Xiaoyaosan can significantly improve the learning and memory abilities of AD mice, which may be related to the reduction of cognitive impairment in AD mice by regulating abnormal deposition and degradating Aβ in the hippocampus.
