Effect of Shugan Wendan Decoction on LXRα, NF-кB and Endothelial Function in Atherosclerosis Rabbits
10.13422/j.cnki.syfjx.20191639
- VernacularTitle: 疏肝温胆汤对动脉粥样硬化家兔LXRα，NF-кB及内皮功能的影响
- Author:
Shuai ZHAO
1
;
Lie-yuan ZHANG
1
;
Wen-wei FENG
1
;
Wei-zhang ZHANG
1
;
Yong-ning GUO
1
;
Zi-cong ZHAO
1
;
Bo-jun CHEN
1
Author Information
1. The Second Clinical Medicine School of Guangzhou University of Chinese Medicine, Guangzhou 510006, China
- Publication Type:Research Article
- Keywords:
Shugan Wendan decoction;
atherosclerosis;
liver X receptor α;
nuclear factor-κB;
signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):108-115
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of Shugan Wendan decoction in treating atherosclerosis based on liver X receptor α(LXRα)/nuclear factor-κB(NF-κB) signal. Method:A New Zealand rabbit model of atherosclerosis with liver-Qi stagnation was established by using calf serum albumin immune injury, high fat feeding and bondage emotional stress method. Theses rabbits are randomly divided into 6 groups, control group,model group, atorvastatin group, Shugan Wendan decoction low, medium and high dose group(2.18,6.54,19.62 g·kg-1·d-1). After successful modeling, the rabbits were treated by injecting drugs with Atorvastatin and low, middle and high dose Shugan Wendan decoction to gastric.The control group and the model group were given intragastric administration of saline in the same volume. The period of gavage is 6 weeks. The pathological changes of the rabbits were detected by hematoxylin-eosin(HE) staining.Serum levels of totalcholesterol(TC), triglyceride(TG),low density extremityprotein(LDL-C), high density extremity protein(HDL-C), nitric oxide(NO), and endothelin-1(ET-1) of the rabbits were detected by enzyme method, nitrate reductase method, and enzyme-linked immunosorbent assay(ELISA), respectively.The gene expression of CRP, IL-1β, IL-6 and MMP-9 in the aorta was detected by Real-time fluorescent quantitative polymerase chain reaction(Real-time PCR) method.The protein expression of LXRα/NF-κB signaling pathway wasdetected by Western blot. Result:Compared with normal control group, in model group, the lumen of the blood vessels was significantly narrowed, atheromatous plaques were formed, and a large number of intracellular foam-like changes were seen. In atorvastatin group and Shugan Wendan decoction group, the blood vessels in high, middle, and low concentration groups were narrowed. Atherosclerotic plaques and foam-like changes were all lower than the model group.Compared with the normal control group, the TG, TC, and LDL-C levels in the model groupincreased(P<0.05), HDL decreased (P<0.05), NO decreased (P<0.05),ET-1 increased (P<0.05),the expression levels of hs-CRP, IL-1β, IL-6 and MMP-9 all increased(P<0.05), the expression of LXRα protein in the model group was decreased(P<0.05), and the protein expression of NF-κB was increased(P<0.05). Compared with model group,the levels of TG, TC and LDL-C the atorvastatin group andlow, medium, and highdose Shugan Wendan decoction group decreased,(P<0.05),NO in the atorvastatin group and the highdose Shugan Wendan decoction group increased and ET-1 decreased(P<0.05).In group comparison, the expression levels of CRP, IL-1β, IL-6 and MMP-9 in the atorvastatin group,the low, middle and high dose Shugan Wendan decoction groups all decreased(P<0.05).The expression of LXRα protein in the group was increased(P<0.05) and the protein expression of NF-κB was decreased(P<0.05). Conclusion:Shugan Wendan decoction can inhance the function of vascular endothelial cells and the stability of atherosclerotic plaque by regulating LXRα/NF-κB signaling pathway.
