Effect of Modified Sijunzi Tang on Protein and mRNA Expressions of Occludin, ZO-1 and Claudin-1 in Cerebral Ischemia Rats
10.13422/j.cnki.syfjx.20191201
- VernacularTitle: 加味四君子汤对脑缺血大鼠脑组织Occludin，ZO-1，Claudin-1蛋白及其mRNA表达的影响
- Author:
Bing-rong TANG
1
;
Hua LI
1
;
Wang-hua LIU
1
;
Kang-li HU
1
Author Information
1. Key Laboratory of Diagnostics of Chinese Medicine in Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China
- Publication Type:Research Article
- Keywords:
cerebral ischemia;
brain edema;
modified Sijunzi Tang;
invigorating spleen and replenishing Qi and draining water;
Occludin;
Claudin-1;
zonula occludens-1(ZO-1)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):57-63
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of modified Sijunzi Tang on protein and mRNA expressions of Occludin, Claudin-1 and zonula occludens-1(ZO-1) in cerebral ischemia rats. Method:Totally 60 male Sprague-Dawley rats were randomly divided into sham operation group, model group, edaravone group, small-dose modified Sijunzi Tang group, middle-dose modified Sijunzi Tang group and high-dose modified Sijunzi Tang group. The middle cerebral artery occlusion (MCAO) model was prepared by suture method. After 7 days of treatment, the modeling group was put to death. Western blot was used to detect the contents of Occludin, Claudin-1 and ZO-1 in the ischemic cerebral cortex of rats. Detection of Occludin, ZO-1, Claudin-1 mRNA expression in the ischemic cortex of rats by Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR). Result:Compared with the sham operation group, protein expressions of Occludin, Claudin-1, and ZO-1 in the model group were significantly down-regulated (P<0.01). Compared with the model group, protein expressions of Occludin, ZO-1,Claudin-1 in the positive control group and modified Sijunzi Tang groups increased significantly, with statistically significant differences (P<0.01).Compared with the Sham operation group, the expression of Occludin, Claudin-1, and ZO-1 mRNA in the model group was down-regulated (P<0.01). Compared with the model group, mRNA expressions of Occludin, Claudin-1, ZO-1 in the positive control group and modified Sijunzi Tang groups increased, with statistically significant differences (P<0.01). Conclusion:Modified Sijunzi Tang may protect the blood-brain barrier and reduce brain edema in ischemic stroke rats by increasing the expression of tight junction proteins Occludin, ZO-1 and Claudin-1.
