Separation and Purification of Costunolide and Dehydrocostus Lactone in Aucklandiae Radix by Supercritical Fluid Chromatography
10.13422/j.cnki.syfjx.20191011
- VernacularTitle: 超临界流体色谱分离纯化木香中的木香烃内酯和去氢木香内酯
- Author:
Yun-yun LIU
1
;
Lin-lin YU
1
;
Xiao-yan LIU
1
;
Ai-feng LI
1
;
Ai-ling SUN
1
;
Ren-min LIU
1
Author Information
1. School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252000, China
- Publication Type:Research Article
- Keywords:
supercritical fluid chromatography;
Aucklandiae Radix;
purification;
costunolide;
dehydrocostus lactone
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):179-185
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a supercritical fluid chromatography(SFC) method for separating and purifying costunolide and dehydrocostus lactone in Aucklandiae Radix. Method:With supercritical carbon dioxide as the mobile phase,the effect of six factors, such as type of chromatographic columns,modifiers and modifiers ratio, flow rate of mobile phase,pressure and temperature, on the separation process of supercritical fluid chromatography were explored. The target components were separated and prepared by semi-preparative supercritical fluid chromatography. High performance liquid chromatography and nuclear magnetic resonance were used to analyze the components and study the thermodynamic regularity of the chromatographic process. Result:C18 column (10 mm×250 mm,5 μm) was adopted, with supercritical fluid dioxide as the mobile phase,the ratio of methanol was 0.13%,the flow rate was 12 mL·min-1,column pressure was 13 MPa,column temperature was 318℃, and detection wavelength was 225 nm. The sample was injected for 20 times,crude extract was 4 mg,and each target component was collected according to the chromatogram. Its purity was determined to be more than 99%by HPLC,and its structure was determined as costunolide and dehydrocostus lactone by NMR. Under this condition,the SFC separation process was normal-phase chromatography. Conclusion:The method can be used to prepare effective components of Aucklandiae Radix with a high purity and low solvent residue.
