Isolation and Identification of Chemical Constituents from Ethanol Extract of Camptosorus sibiricus
10.13422/j.cnki.syfjx.20191413
- VernacularTitle: 过山蕨乙醇提取物化学成分的分离鉴定
- Author:
Ling-yue LYU
1
;
Dai-zhou ZHANG
2
;
Fang WANG
2
;
Qing-wen JIA
2
;
Da-lian LIANG
2
;
Min LI
2
;
Wei ZHANG
3
Author Information
1. Shandong University of Traditional Chinese Medicine(TCM), Ji'nan 250355, China
2. Shandong Academy of Pharmaceutical Sciences, Ji'nan 250101, China
3. Qingdao Hospital of TCM, Qingdao 266033, China
- Publication Type:Research Article
- Keywords:
Camptosorus sibiricus;
95% ethanol extract;
chemical constituents;
structure identification;
antioxidant activity
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):168-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the chemical constituents in 95%ethanol extract of the whole plant of Camptosorus sibiricus and determine its antioxidant activity. Method:Compounds were isolated by a combination of various chromato-graphic techniques, including column chromatography over silica gel and Sephadex LH-20 and reversed-phase HPLC. Their structures were identified on the basis of physicochemical properties and spectral data reported in the literature. Result:Eleven compounds were identified as trans-p-coumaric acid (1),trans-p-coumaric acid 4-O-β-D-glucoside (2),cis-p-coumaric acid 4-O-β-D-glucoside (3),(E)-ferulic acid-4-O-β-D-glucoside (4),caffeic acid methyl ester (5),ferulic acid methyl ester (6),syringic acid (7),syringic acid-4-O-β-D-glucopyranoside (8),protocatechualdehyde (9),vanillain (10) and syringaldehyde (11),respectively. Conclusion:Compounds 3-11 are isolated from the genus Camptosorus for the first time. In the in vitro SOD-like activity assays,compounds 7,9-11 show an antioxidant activity with half maximal inhibitory concentration(IC50)values of 16.70,11.70,12.23 and 13.52 μmol·L-1,respectively.
