Metabonomics Analysis of Sailuotong Capsules in Treatment of Acute Cerebral Ischemia in Rats
10.13422/j.cnki.syfjx.20191552
- VernacularTitle: 塞络通胶囊治疗急性脑缺血模型大鼠的代谢组学分析
- Author:
Cong ZHANG
1
;
Jian-xun LIU
1
;
Ming-qian SUN
1
;
Ye-hao ZHANG
1
;
Chang-ying REN
1
;
Chun-yuan YIN
1
Author Information
1. Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
- Publication Type:Research Article
- Keywords:
Sailuotong capsules;
acute cerebral ischemia;
metabonomics;
biomarkers;
metabolic pathways;
N-acetylaspartate metabolism;
arginine metabolism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):136-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of Sailuotong capsules in treating acute cerebral ischemia from the perspective of metabonomics. Method:A total of 24 SD rats were randomly divided into 3 groups, including sham-operated group, model group and Sailuotong group (33 mg·kg-1). The rat model of acute multiple cerebral infarction was established by injecting fluorescent microspheres into internal carotid artery. After the successful operation, rats in Sailuotong group were administered by duodenal injection immediately, and the dosage volume was 2 mL·kg-1. Endogenous metabolites in rat brain tissues of each group were determined by UPLC-Q-TOF-MS. The relevant data and biomarkers were analyzed by principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). Result:The analysis of pattern recognition indicated that the metabolite profiles in model group and sham-operated group were separated obviously, and ten biomarkers related to acute cerebral ischemia were also identified. Compared with the sham-operated group, contents of N-acetylaspartate (NAA), fumaric acid, glutathione, dehydroascorbic acid, aspartic acid and S-adenosylhomocysteine were decreased, while the contents of arginine, citrulline, saccharopine and hydantoin-5-propionic acid were increased in the model group. Meanwhile, the ten abnormal biomarkers mentioned above got restoration in Sailuotong group. Conclusion:The main regulated metabolic pathways of Sailuotong capsules are NAA metabolism, arginine metabolism, energy metabolism, oxidative stress, etc.
