Effect of Sishenwan on Toll-like Receptor 4 and IRAK-M Expression in Colonic Tissue of Rats with Ulcerative Colitis of Spleen-kidney Yang Deficiency Type
10.13422/j.cnki.syfjx.20191439
- VernacularTitle: 四神丸对脾肾阳虚型溃疡性结肠炎模型大鼠结肠组织Toll样受体4及其负性调控因子IRAK-M表达的影响
- Author:
Ai-hua WANG
1
;
Lan-juan HE
2
;
Xiang-dong ZHU
2
Author Information
1. Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China
2. Gansu University of Chinese Medicine, Lanzhou 730000, China
- Publication Type:Research Article
- Keywords:
Toll-like receptor 4(TLR4);
interleukin-1 receptor-associated kinase-M(IRAK-M);
ulcerative colitis;
Sishenwan;
mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):70-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the Toll-like receptor 4(TLR4)and its negative regulating factorInterleukin-1 receptor-associated kinase-M(IRAK-M)in colonic mucosa of rats with experimental ulcerative colitis(UC), and to discuss the mechanism of the Chinese medicine Sishenwan. Method:The 90 Wistar rats were randomly divide into six groups, blank group, model group, sulfasalazine group(0.36 g·kg-1), Sishenwan low, medium and high-dose group(2.5, 5, 10 g·kg-1), 15 cases in each group. A rat model of UC was prepared by using a solution of trinitrobenzenesulfonic acid/ethano.The histopathological changes of colon were observed by hematoxylin-eosin (HE) staining. The contents of serum free triiodothyroid acid (FT3), serum free thyroxine (FT4), immunoglobulin (Ig) E and interleukin (IL)-2 were determined by radioimmunoassay. The activity of superoxide dismutase (SOD) in rat serum was determined by xanthine oxidation method. The activity of malondialdehyde (MDA) in serum of rats was determined by thiobarbituric acid (TBA) colorimetry. Result:Compared with blank group, intestinal mucosal injury score of rats in model group was significantly increased (P<0.01), serum IgE and MDA contents were significantly increased (P<0.01).The expression levels of FT3, FT4, IL-2 and SOD were significantly decreased (P<0.01).The TLR4 mRNA and protein expression in model group increased significantly(P<0.01).The expression of IRAK-M mRNA and protein decreased significantly(P<0.01).Compared with model group, scores of each treatment group were significantly decreased (P<0.01), IgE and MDA contents were significantly decreased (P<0.05, P<0.01), FT3, FT4, IL-2, and SOD contents were significantly increased (P<0.05, P<0.01).The expression of TLR4 mRNA and proteinin each treatment group was significantly reduced(P<0.05, P<0.01), IRAK-M mRNA and protein expression level increased(P<0.01). Conclusion:The unbalanced expressions of TLR4 and its negative regulating factor IRAK-M are connected with the pathogenesis of UC.Sishenwan can cure UC and control the expression of TLR4 and promote the expression of IRAK-M.
