Analysis on Quality Transmitting of Substance Benchmarks of Shaoyao Gancaotang
10.13422/j.cnki.syfjx.20190946
- VernacularTitle: 经典名方芍药甘草汤的物质基准量值传递分析
- Author:
Xiao-zhang TANG
1
;
Mei-si LIN
2
;
Fei ZHOU
1
;
Lin WANG
1
;
Yuan-yuan LI
3
;
Jian-jun TANG
3
;
Da-sheng LIN
1
;
Xing GAO
3
Author Information
1. School of Pharmacy, Key Laboratory of Traditional Chinese Medicine(TCM) Resources with Southwest Characteristics, Chengdu University of TCM, Chengdu 611137, China
2. School of Acupuncture and Moxibustion, Affiliated Hospital of Chengdu University of TCM, Chengdu 610097, China
3. Chengdu Taihe Health Technology Group Co. Ltd., Chengdu 611731, China
- Publication Type:Research Article
- Keywords:
famous classical formulas;
Shaoyao Gancaotang;
substance benchmarks;
fingerprint;
quality transmitting;
paeoniflorin;
liquiritin
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):62-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the HPLC fingerprint test method for the medicinal materials, decoction pieces and substance benchmarks of Shaoyao Gancaotang for investigating the quality transmitting of substance group in preparation process of the medicinal materials-decoction pieces-substance benchmarks, then to evaluate the scientificity and rationality of preparation process of substance benchmarks of Shaoyao Gancaotang by combining with yields of dry extract, transfer rates of effective components and other indexes. Method:Substance benchmarks of Shaoyao Gancaotang was prepared according to the method recorded in ancient medical books, fingerprints of 15 batches of medicinal materials, decoction pieces and substance benchmarks were detected by HPLC, and the contents of effective ingredients were determined. At the same time, the correlation analysis of quality transmitting of substance group during the preparation of substance benchmarks was carried out by combining the yields of dry extract and transfer rates of effective components. Result:The established HPLC fingerprint method has good precision, repeatability and stability, it can be used for the simultaneous determination of fingerprint of medicinal materials, decoction pieces and substance benchmarks. In the fingerprint of substance benchmarks, 16 common peaks were determined by taking liquiritin as the reference peak, of which 6 chromatographic peaks belong to Paeoniae Radix Alba and 11 chromatographic peaks belong to Glycyrrhizae Radix et Rhizoma, 7 major chromatographic peaks were identified. The similarities of fingerprints of 15 batches of medicinal materials, decoction pieces and substance benchmarks of Shaoyao Gancaotang were good by comparing with their respective reference fingerprints(≥ 0.90), the average dry extract rate of 15 batches of substance benchmarks was 24.81%, and no discrete data were found; the average transfer rates of paeoniflorin, liquiritin and glycyrrhizic acid from decoction pieces to substance benchmarks were 79.68%, 63.70% and 51.20%, respectively, and no discrete data were found. Conclusion:In this paper, a scientific and reasonable method for evaluating the process of substance benchmarks of Shaoyao Gancaotang is established by means of the fingerprint method controlled by the whole substance group, the research idea of quality transmitting of substance group in the preparation process, and the evaluation of technical and economic indicators. It can be used as a reference for the evaluation and research of material benchmarks in other famous classical formulas.
