Effect of Fengshi Qutong Capsule on Synovial Akt and MAPK Signaling Pathways in Rheumatoid Arthritis
10.13422/j.cnki.syfjx.20191336
- VernacularTitle: 风湿祛痛胶囊对类风湿关节炎滑膜Akt和MAPK信号通路的影响
- Author:
Chun-fang LIU
1
;
Lian-hua HE
1
;
Jing-xia WANG
1
;
Yi-qun LI
1
;
Cong-cong SUN
1
;
Yu JING
2
;
Yan-dong MIAO
2
;
Na LIN
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences, Beijing 100700, China
2. Tonghua Golden-horse Group, Beijing 100028, China
- Publication Type:Research Article
- Keywords:
Fengshi Qutong capsule;
protein kinase B;
mitogen-activated protein kinase;
collagen-induced arthritis;
fibroblast-like synovial cell;
endothelial cell;
rheumatoid arthritis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(13):35-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Fengshi Qutong capsule (FSQTC) on protein kinase B(Akt) and mitogen-activated protein kinase (MAPK) signaling pathways of rheumatoid arthritis (RA). Method:Collagen-induced arthritis (CIA) was induced in SD rats, and the synovial membranes of the knee joints were prepared after 19 days of oral administration of 0.25, 0.5, 1 g·kg-1 FSQTC. MH7A cells were induced by tumor necrosis factor-α (TNF-α, 20 μg·L-1) in vitro, and human umbilical vein endothelial cells (HUVEC) were induced by vascular endothelial growth factor (VEGF). FSQTC (0.02, 0.1, 0.5 μg·L-1) were added to MH7A/HUVEC cells, and then the cells were collected. Proteins of synovial tissue, MH7A and HUVEC cells were extracted, and then were detected the expresstion of p-Akt, p-p38 MAPK, p-extracellular signal-regulated kinase(ERK) and p-Jun n-terminal kinase(JNK) by Western blot. Result:The expression levels of p-Akt, p-p38 MAPK, p-ERK and p-JNK in the synovial membrane of CIA model were significantly increased compared with normal group (P<0.01), and the treatmend of 0.25, 0.5 and 1 g·kg-1·d-1 FSQTC significantly decreased their expression levels (P<0.05, P<0.01). To compared with control group, the expression levels of p-Akt, p-p38 MAPK, p-ERK and p-JNK in MH7A or HUVEC cells induced by TNF-α or VEGF were increased (P<0.01), respectively, and these factors are significantly reduced by 0.02, 0.1, 0.5 μg·L-1 FSQTC (P<0.05, P<0.01). Conclusion:FSQTC can down-regulate the abnormal activation of Akt and MAPK signaling pathways in the synovial membrane of CIA rats, fibroblast synovial cells and vascular endothelial cells, which is related to the inhibition of synovial angiogenesis in the treatment of RA.
