Effect of Sinisan on Macrophage Polarization of RAW264.7 Cells Induced by Lipopolysaccharide
10.13422/j.cnki.syfjx.20191301
- VernacularTitle: 四逆散对脂多糖诱导的RAW264.7细胞极化的影响
- Author:
Hui-jie FAN
1
;
Zhang-bin TAN
1
;
Hong-feng LIANG
2
;
Bin LIU
3
;
Xiao-shan ZHAO
1
;
Ying-chun ZHOU
1
Author Information
1. School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China
2. Yangjiang People's Hospital, Yangjiang 529500, China
3. The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China
- Publication Type:Research Article
- Keywords:
Sinisan;
lipopolysaccharide (LPS);
RAW264.7;
polarization of macrophage;
inflammation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(13):9-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulatory effect of Sinisan(SNS) on the polarization of RAW264.7 macrophages induced by lipopolysaccharide (LPS). Method:RAW264.7 cells stimulated by LPS were used as the in vitro model. The cells were intervened with the different concentrations of SNS in advance. The effects of different concentrations of SNS on the proliferation of RAW264.7 cells were detected by methyl thiazolyl tetrazolium (MTT) colorimetry. The degree of cell differentiation was detected by enzyme-linked immuno sorbent assay(ELISA) method. The contents of M1 polarization factors tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β) and M2 polarization factors interleukin-10 (IL-10) in cell culture supernatant were detected by ELISA method, mRNA levels of M1 polarization factors TNF-α, IL-6 and M2 polarization factors IL-10, arginase-1 (Arg-1) were detected by Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR) method. Result:SNS had no effect on the cell viability of RAW264.7 cells, inhibited LPS-induced cell proliferation, decreased LPS-stimulated cell differentiation, down-regulated M1 polarizing factors TNF-α, IL-6, IL-1β release and TNF-α, IL-6 mRNA levels, and increased the release of IL-10 and mRNA levels of IL-10 and Arg-1. Conclusion:SNS inhibits the inflammation of RAW264.7 cells induced by LPS, and its mechanism may be related to the regulation of polarization balance of M1/M2 macrophages.
