Determination of Content Limit of Isoflavone Glucoside of Hengshan Wild-simulated Astragali Radix by ROC Curve Method
10.13422/j.cnki.syfjx.20191311
- VernacularTitle: ROC曲线方法确定恒山仿野生黄芪毛蕊异黄酮葡萄糖苷的含量限度
- Author:
Qing-wei CAO
1
;
Rui ZHANG
1
;
Ke LI
1
;
Ai-ping LI
1
;
Yue-tao LIU
1
;
Xue-mei QIN
1
Author Information
1. Modern Research Center of Traditional Chinese Medicine(TCM), Shanxi University, Taiyuan 030006, China
- Publication Type:Research Article
- Keywords:
Astragali Radix;
isoflavones glucoside;
ROC curve;
method analysis;
content limit
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(12):158-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the receiuer operating characteristic(ROC) curve mathematical statistics method to establish the content limit of isoflavones glucoside contents in Astragali Radix,in order to distinguish the difference in isoflavones glucoside content between Hengshan wild-simulated Astragali Radix in Shanxi and cultivated Astragali Radix in Gansu. Method: A total of 225 samples of 45 batches of wild-simulated Astragali Radix in Hengshan and 210 samples of 42 batches of cultivated Astragali Radix in Gansu were selected as research objects,and isoflavones glucoside contents of these samples were determined by using the method in the edition 2015 Chinese Pharmacopoeia. Data was statistically analyzed, and the ROC curve was used to determine the critical value of wild-simulated Astragali Radix in Hengshan and transplanted Astragali Radix in Gansu. Result: The average contents of isoflavones glucoside in imitative wild Astragali Radix in Henshan and cultivated Astragali Radix in Gansu were 0.107%and 0.039%respectively,with statistically significant differences(P<0.01). The ROC curve had a critical value of 0.069%,and its sensitivity and specificity was 100%and 93.33%,and its model diagnostic accuracy was 0.978,which indicated accurate prediction results. Conclusion: ROC curve can be used to distinguish the critical value of the wild-simulated Astragali Radix in Hengshan and cultivated Astragali Radix in Gansu. The results reflected the regularity of "high quality and good price" in the market. The research provided a theoretical basis for the establishment of standard of high-quality Chinese herbal medicines.
