Effect of Dry-cold Environment on Metabolic Components in Urine and Plasma of Rats
10.13422/j.cnki.syfjx.201906
- VernacularTitle: 干寒环境对大鼠尿液与血浆代谢成分的影响
- Author:
Aheerzhama-ni MAIMAITI
1
;
Mirea-yi YALIKUN
1
;
Tuer-hong TAILAITI
1
;
Dina-la QIAREFUHAN
1
;
Batur MAMTIMIN
1
Author Information
1. Central Laboratory, School of Pharmacy, Institute of Traditional Uighur Medicine, Xinjiang Medical University, Urumqi 830011, China
- Publication Type:Research Article
- Keywords:
dry-cold environment;
diet;
metabolomics;
nuclear magnetic resonance hydrogen spectrum(1H-NMR);
tricarboxylic acid cycle;
amino acids;
metabolic pathways
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(11):115-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Nuclear magnetic resonance hydrogen spectrum(1H-NMR) was adopted to investigate the effect of dry-cold environment and diet on urine and plasma metabolites of rats,in order to find possible biomarkers and their related metabolic pathways. Method:The dry-cold environment and diet were used as a stressors to intervene rats for 3 weeks,urine and plasma samples were collected.1H-NMR combined with multivariate data analysis were used to identify relevant metabolic markers and analyze their metabolic pathways. Result:The dry-cold environment and diet resulted in significant changes of 23 metabolites in urine and 15 metabolites in plasma;these metabolites were closely related to changes of various metabolic pathways,namely the tricarboxylic acid(TCA) cycle,pyruvate metabolism,glycolysis or gluconeogenesis,valine,leucine and isoleucine biosynthesis,glycine,serine and threonine metabolism,histidine metabolism;glyoxylic acid and dicarboxylic acid metabolism,alanine,aspartic acid and glutamate metabolism. Conclusion:Dry-cold environment and diet conditions can lead to the changes of various metabolic pathways in the body.The metabolomics based on 1H-NMR has the advantage of explaining the pathophysiological dynamics and overall changes of the body,which is of great significance for exploring the pathogenesis of non-deterministic disease factors.
