Mechanism of Nephrotoxicity of Aloe Emodin in Mice
10.13422/j.cnki.syfjx.20190424
- VernacularTitle: 芦荟大黄素对小鼠肾毒性的作用机制
- Author:
Yan-qiao LI
1
;
Wan-yi HUANG
1
;
Yu-sheng LIANG
1
;
Yu LUO
1
;
Qing JIANG
1
;
Yong ZENG
1
;
Ping WANG
1
;
Xian-li MENG
1
Author Information
1. Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
- Publication Type:Research Article
- Keywords:
aloe-emodin;
nephrotoxicity;
oxidative stress;
apoptosis;
transforming growth factor-β1 (TGF-β1)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(11):48-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study nephrotoxicity induced by long-term administration of different doses of aloe-emodin in mice, and explore its mechanism. Method:A total of 30 male and female Kunming mice were randomly divided into normal control group, and low-dose aloe-emodin group,high-dose aloe-emodin group (0.8,1.6 g·kg-1). Every dose of group was administered intragastrically for 11 weeks,twice daily. effect of serum urea nitrogen (BUN),creatinine (SCr),superoxide dismutase (SOD),malondialdehyde (MDA),Glutathione (GSH/GSSG) and Glutathione Peroxidase (GSH-Px) levels were detected by biochemical kits according to manufacturer's instruction. Enzyme-linked immune assay was used to determine serum tumor necrosis factor (TNF)-α and interleukins(IL)-6 levels. Hematoxylin eosin (HE) staining was used to detect renal pathological changes in kidney tissues, and cysteine aspartic acid specific protease(Caspase)-3 and transforming growth factor(TGF)-β1 proteins were determined by immunohistochemistry. Result:According to results,compared with normal control group,the levels of BUN and SCr in serum with high-dose aloe-emodin were increased. The renal tubules in low-dose group were mildly injured,while renal tubules and glomeruli of high-dose group were moderately damaged. Compared with normal control group,the level of SOD was significant decreased (P<0.05), MDA was increased (P<0.05),the levels of GSH/GSSG in kidneys of high-dose groups were decreased (P<0.05). In high-dose group,the expression of Caspase-3 protein was increased in kidneys,especially in males (P<0.05). Compared with normal control group,the levels of TNF-α and IL-6 were increased,the expression of TGF-β1 protein in kidneys was increased in low-dose and high-dose groups (P<0.05). Conclusion:results show that 1.6 g·kg-1 aloe-emodin was administered intragastrically for 11 weeks,which had toxic effects on kidney in mice. The mechanism may be related to oxidative stress,apoptosis and TGF-β1 protein expression.
