Effect of Compatibility of Alcohol Extract of Magnoliae Officinalis Cortex and Alcohol Extract of Polygalae Radix on Fecal Metabolites of Rats
10.13422/j.cnki.syfjx.20190303
- VernacularTitle: 厚朴醇提物与远志醇提物配伍对大鼠粪便代谢物的影响
- Author:
Rong MA
1
;
Qian XIE
1
;
Jian WANG
1
;
Li-hua HUANG
1
;
Xiao MA
1
;
Nian CHEN
1
;
Tai-wei DONG
1
;
Xiao-qing GUO
1
;
Ya-mei FAN
1
Author Information
1. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese Herbal Medicine, Ministry of Education, Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province, Key Laboratory Breeding Base of Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China
- Publication Type:Research Article
- Keywords:
Magnoliae Officinalis Cortex;
Polygalae Radix;
compatibility;
feces;
metabolic markers;
tryptophan metabolism;
primary bile acids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(11):1-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of alcohol extract of Magnoliae Officinalis Cortex,alcohol extract of Polygalae Radix and their compatibility on fecal metabolites of rats,analyze its potential metabolic pathways,and provide experimental basis for exploring the possible mechanism of Magnoliae Officinalis Cortex relieving gastrointestinal motility disorders induced by Polygalae Radix. Method:Forty male SD rats were randomly divided into the normal group,alcohol extract of Magnoliae Officinalis Cortex group(3.50 g·kg-1),alcohol extract of Polygalae Radix group(1.75 g·kg-1) and compatibility group (3.5 g·kg-1 of alcohol extract of Magnoliae Officinalis Cortex+1.75 g·kg-1 of alcohol extract of Polygalae Radix).Fecal samples were collected within 24 h after continuous gavage for 3 days.The fecal metabolites in each group was detected by ultra-high performance liquid chromatography-quadrupole-time of flight-mass spectrometry(UPLC-Q-TOF-MS),mobile phase was acetonitrile-0.1%formic acid solution for gradient elution,data collection range was m/z 50-1 200 under positive and negative ion mode of electrospray ionization.The characteristic biomarkers and corresponding metabolic pathways were analyzed or screened by Progenesis QI v2.0,SIMCA-P 14.0,SPSS 20.0,MetaboAnalyst 4.0 and other softwares. Result:A total of 17 characteristic metabolic markers were screened out,including 5-formiminotetrahydrofolic acid,L-3-hydroxykynurenine,7,8-dihydropteroic acid,etc.The main related pathways included biosynthesis of unsaturated fatty acids,linoleic acid metabolism,vitamin B6 metabolism,etc. Conclusion:The mechanism of Magnoliae Officinalis Cortex relieving gastrointestinal motility disorders induced by Polygalae Radix may be related to purine metabolism,folate biosynthesis,tryptophan metabolism and primary bile acid biosynthesis.
