Effect of Tianlong Tongxin Tablet on Myocardial Ischemia Reperfusion Injury and Hemorheology
10.13422/j.cnki.syfjx.20191005
- VernacularTitle: 天龙通心片对心肌缺血再灌注损伤及血液流变学的影响
- Author:
Ling-mei LI
1
;
Jian-hua FU
1
;
Cheng-ren LIN
2
;
Xiao-bin MA
2
;
Yang-hui WANG
2
;
Min WANG
2
;
Lei LI
2
;
Hao GUO
2
;
Xiao HAN
2
;
Jian-xun LIU
2
Author Information
1. Binzhou Medical University, Yantai 264003, China
2. Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
- Publication Type:Research Article
- Keywords:
Tianlong Tongxin tablets;
myocardial ischemia reperfusion injury;
hemorrheology
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(10):41-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the protective effect of Tianlong Tongxin tablet on myocardial ischemia reperfusion injury in rats, and observe its effect on thrombosis, blood viscosity and platelet aggregation in rabbits. Method:Totally 56 Wistar rats were collected. Except for the sham operation group, all of the remaining rats were involved in the establishment of the rat myocardial ischemia reperfusion injury model. The successfully established model was divided model group, Hexinshuang group, compound Danshen tablet group and Tianlong Tongxin tablet groups (4, 2, 1 g·kg-1). Nitrotetrazolium blue (N-BT) method was used to observe the alleviation of myocardial infarction. Colorimetry was used to detect the effect of the test drug on serum superoxide dismutase (SOD) and malondialdehyde (MDA). The Chandler in vitro method was used to detect thrombosis and blood viscosity in vitro of control group, Tianlong Tongxin tablets groups (4, 2, 1 g·kg-1), compound Danshen tablets group and aspirin group. The Born turbidimetric method was used to observe the platelet aggregation levels of control group, Tianlong Tongxin tablets groups (2, 1, 0.5 g·kg-1), compound Danshen tablets group and aspirin group. Result:Compared with the sham operation group, the myocardial infarction area, serum SOD and MDA in the model group were significantly increased (P<0.05). Compared with the model group, Tianlong Tongxin tablets groups, Hexinshuang group and compound Danshen tablets group could reduce the degree of myocardial infarction, and the percentage of infarction area in the ventricle and heart decreased significantly (P<0.05, P<0.01). Compared with the control group, Tianlong Tongxin tablets groups (4, 2 g·kg-1), compound Danshen tablets group and Aspirin tablets group could significantly shorten the length of thrombosis (P<0.05, P<0.01), and significantly reduce the wet weight and dry weight of thrombosis (P<0.05, P<0.01). In addition, the whole blood viscosities under 150, 60 and 5 s-1 shear rates were significantly reduced (P<0.05, P<0.01). Compared with the control group, the platelet aggregation rates induced by collagen, adenosine bisphosphate (ADP) and arachidonic acid (AA) in rabbits were significantly reduced in Tianlong Tongxin tablet group (2, 1 g·kg-1), compound Danshen tablet group and Aspirin tablet group (P<0.05, P<0.01). Conclusion:Tianlong Tongxin tablet can protect rat myocardial ischemia reperfusion injury, inhibit platelet aggregation and thrombosis, and reduce blood viscosity.
