Analysis of Main Components in Asarum heterotropoides var. mandshuricum Seedling at Different Growth Periods and Maturation Period
10.13422/j.cnki.syfjx.20190309
- VernacularTitle: 北细辛苗在不同生长阶段与成熟期的主要化学成分分析
- Author:
Ya-nan LU
1
;
Xiao-qing TIAN
1
;
Xiao-yun ZHOU
2
;
Yu-bao QIAO
1
;
Ying-ying TANG
1
;
Li-yan MA
1
;
Cheng-qi FAN
1
Author Information
1. Key Laboratory of Oceanic and Polar Fisheries, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
2. Jilin Hospital of Integrated Traditional Chinese and Western Medicine in Jilin Province, Jilin 132010, China
- Publication Type:Research Article
- Keywords:
Asarum heterotropoides var. mandshuricum;
seedling;
growth stages;
aerial part;
underground part;
secondary metabolites;
asarinin
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(9):159-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the change rule of main chemical components in Asarum heterotropoidesvar.mandshuricum seedling during the growing process.Method:Whole seedling samples (one week and two weeks) and the mature plant (three months) of A.heterotropoidesvar.mandshuricum were collected and every sample was divided to aerial part (stems and leaves) and underground part (roots).The secondary metabolites were qualitatively identified by HPLC-TOF-MS and the quantitative identification was carried out at the same time with asarinin as index component.Result: A total of 6 known compounds were identified from the underground part of A.heterotropoidesvar.mandshuricum as α-asarone (1),N-isobutyl-2,4,8,10-dodecatetraenamide (2),9-methoxyaristolactam Ⅳ(3),asarinin (4),caulesnarinside (6) and chalcononaringenin 2',4'-di-O-β-D-glucopyranoside (7),respectively,the peak area values showed that the contents of these compounds increased gradually with the growth time.A total of 4 known compounds were identified from the aerial part of this herb as N-isobutyl-2,4,8,10-dodecatetraenamide (2),caulesauroneside (5),caulesnarinside (6) or chalcononaringenin 2',4'-di-O-β-D-glucopyranoside (7) and peonidin 3-caffeoylgentiobioside (8).Asarinin was identified only in the underground part of mature plant,its content was 155.4 μg·g-1.Conclusion: The species and contents of secondary metabolites are quite different in the aerial and underground parts of A.heterotropoidesvar.mandshuricum.At different growth stages of A.heterotropoides var.mandshuricum seedling,the types and contents of secondary metabolites in the same site are also different,while the contents of main components show an increasing trend with the growth time.
