Effect of Yiqi Huoxue Recipe on Vascular Endothelial Function in Rats with Qi Deficiency and Blood Stasis Syndrome
10.13422/j.cnki.syfjx.20190838
- VernacularTitle: 益气活血方对气虚血瘀证大鼠血管内皮功能的影响
- Author:
Ting-ting HUANG
1
;
Xiao HAN
2
;
Jian-xun LIU
2
;
Ya-hong SHI
2
;
Lei LI
2
;
Chun-yuan YIN
1
;
Jun-guo REN
2
Author Information
1. Graduate School, Beijing University of Chinese Medicine, Beijing 100029, China
2. Beijing Key Laboratory of Pharmacology of Traditional Chinese Medicine, Institute of Basic Medical Sciences, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
- Publication Type:Research Article
- Keywords:
sleep deprivation;
Qi deficiency and blood stasis syndrome;
vascular endothelial cell function;
Yiqi Huoxue Recipe;
Buyang Huanwu Tang
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(8):40-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the changes and benefits of vascular endothelial cell function in rats with qi deficiency and blood stasis syndrome, and the effect of Yiqi Huoxue recipe (YQHXF) of such changes. Method: Rats were randomly divided into blank control group, Qi deficiency and blood stasis group, and YQHXF high and low dose groups (5.54,2.77 g·kg-1). A small platform of water environment was used to make the rats stand for a long-term with irregular and incomplete sleep deprivation, 16 h per day for six weeks, so that both mentality and labor of rats were consumed to establish qi deficiency and blood stasis rat models. From the fifth week, intragastric administration was given for 2 weeks, until end of the experiment. Then levels of endothelin-1(ET-1), von willebrand factor (vWF), vascular cell adhesion molecule-1(VCAM-1), intercellular adhesion molecule (ICAM-1), P-selectin,interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in rat plasma were detected by enzyme-linked immunosorbent assay (ELISA) and nitric oxide (NO) was detected by nitrate reductase assay. Result:Compared with blank control group, rats in Qi deficiency and blood stasis group showed rough and dark hair, with significantly decreased body weight and pulse amplitude (P<0.01); R, G, B values of the tongue surface were decreased significantly (P<0.01); high and moderate shear values of whole blood viscosity, as well as plasma viscosity were significantly increased (P<0.01), and the indexes of vascular endothelial function such as ET-1, vWF, VCAM-1, ICAM-1, P-selectin, IL-6 and TNF-α were abnormally increased after sleep deprivation (P<0.05,P<0.01). High and low dose YQHXF significantly increased the pulse amplitude and tongue R, G, B values(P<0.01), reduced the high, moderate and low shear values of whole blood viscosity, as well as plasma viscosity (P<0.05,P<0.01); at the same time, the levels of ET-1, IL-6, VCAM-1 and P-selectin in rats were decreased (P<0.05, P<0.01). Conclusion:Sleep deprivation can induce the formation of Qi deficiency and blood stasis syndrome in rats, and lead to vascular endothelial dysfunction. YQHXF has the function of protecting the vascular endothelium. It can improve the Qi deficiency and blood stasis symptoms in rats with Qi deficiency and blood stasis syndrome by regulating the secretion of vascular endothelial active substances, reducing cell adhesion and inhibiting inflammation.
