Quality Control of Standard Decoction of Aurantii Fructus Immaturus
10.13422/j.cnki.syfjx.20190102
- VernacularTitle: 枳实饮片标准汤剂的质量控制
- Author:
Jia SHI
1
;
Wen-guang JING
2
;
Zhe DENG
2
;
Meng-jiao JIAO
1
;
Jun ZHANG
1
;
De-wen LIU
1
;
An LIU
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. Institute of Traditional Chinese Medicine(TCM), China National TCM Co. Ltd., Beijing 102600, China
- Publication Type:Research Article
- Keywords:
Aurantii Fructus Immaturus;
standard decoction;
dispensing granules;
quality evaluation;
synephrine;
dry extract rate;
source of Chinese herbs
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(7):153-159
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a quality control method of standard decoction of Aurantti Fructus Immaturus(AFI),and to provide reference for quality evaluation of AFI dispensing granules and other related products of AFI. Method: A total of 16 batches of AFI pieces with different quality were collected from the market,including 13 batches of Citrus aurantium and 3 batches of C. sinensis,and the standard decoction of AFI was prepared according to the standard decoction process.Transfer rate of synephrine,dry extract rate and others of the standard decoction were regarded as evaluation indicators and relative assessment are conducted. Result: Transfer rates of synephrine in 13 batches of standard decoction of AFI(C. aurantium) were ranged from 35.7% to 92.7% with the average value was 61.9%;dry extract rates were varied from 20.7% to 43.8% and the average value was 28.4%;pH values were 4.48-5.32 with the average value was 4.99;the HPLC fingerprint similarities were >0.9 by comparing with the corresponding control fingerprint,6 common peaks were found and 3 of them were identified as naringin,hesperidin and neohesperidin.Transfer rates of synephrine in 3 batches of standard decoction of AFI(C. sinensis) were changed from 53.1% to 84.4%,and the average value was 73.2%;dry extract rates were shifted from 13.8% to 17.6% and the average value was 15.4%;pH values were 4.77-5.38 with the average value was 5.06;the HPLC fingerprint similarities were >0.9 by comparing with the corresponding control fingerprint,2 common peaks were found and one of them were identified as hesperidin. Conclusion: From the HPLC fingerprint of standard decoction of AFI,we can easily understand that the number of peaks in C. aurantium is obviously more than that of C. sinensis.This method has good precision,reproducibility and stability,it is suitable for quality evaluation for related products of AFI.Simultaneously,the research provides a good reference for identifying sources of AFI.
