Effect of Fengshi Qutong Capsule on Function of Human Synovial Cells in Rheumatoid Arthritis Induced by TNF-α
10.13422/j.cnki.syfjx.20190739
- VernacularTitle: 风湿祛痛胶囊对TNF-α诱导的人类风湿关节炎成纤维样滑膜细胞功能的影响
- Author:
Lian-hua HE
1
;
Yi-qun LI
1
;
Jing-xia WANG
1
;
Cong-cong SUN
1
;
Chun-fang LIU
1
;
Yu JING
2
;
Yan-dong MIAO
2
;
Na LIN
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medicine, Beijing 100700, China
2. Tonghua Golden-horse Group, Beijing 100028, China
- Publication Type:Research Article
- Keywords:
Fengshi Qutong capsule;
human synovial cell (MH7A);
proliferation;
migration;
invasion;
adhesion
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(7):116-121
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of Fengshi Qutong capsule (FSQTC) on proliferation, migration, adhesion, invasion and secretion of human synovial cells in rheumatoid arthritis (RA) induced by tumor necrosis factor-α (TNF-α) and explore its mechanism. Method: Human synovial cells (MH7A) in RA patients were induced in vitro by using TNF-α (20 μg·L-1). After treatment with different concentrations of FSQTC (0.02,0.1,0.5 μg·L-1), MTT colorimetric assay, transwell migration, adhesion and invasion tests were used to detect the proliferation, migration, adhesion and invasion of the MH7A, respectively. The expression levels of vascular endothelial growth factor (VEGF) and interleukin-1β (IL-1β) in MH7A supernatant were detected by enzyme linked immunosorbent assay (ELISA). Result: As compared with blank control group, TNF-α (20 μg·L-1) significantly increased the proliferation, migration, adhesion, invasion and secretion of IL-1β and VEGF of MH7A cells (P<0.01). FSQTC (0.02,0.1,0.5 μg·L-1) had no significant effect on proliferation of TNF-α-induced MH7A cells after treatment for 24 hours. After 48 hours of treatment, proliferation of MH7A cells induced by TNF-α was decreased in a concentration-dependent manner (P<0.05,P<0.01). Within 24 hours, the migration, adhesion, invasion, invasion, and secretion of IL-1 and VEGF in MH7A cells were also decreased significantly (P<0.05,P<0.01). Conclusion: FSQTC can inhibit the proliferation, migration, adhesion, invasion and secretion of IL-1β and VEGF in MH7A cells.
