Effect of Capsaicin in Inhibiting Migration and Invasion of Breast Cancer MCF-7 Cells by Down-regulating SIRT1
10.13422/j.cnki.syfjx.20190722
- VernacularTitle: 辣椒碱通过下调SIRT1表达抑制乳腺癌MCF-7细胞的迁移和侵袭
- Author:
An-yun MAO
1
;
Mao-jian CHEN
1
;
Qing-hong QIN
1
;
Chan-chan XIAO
1
;
Wei JIANG
1
;
Zhi-jie LIANG
1
;
Qi-xing TAN
1
;
Wei-ping YANG
1
;
Chang-yuan WEI
1
Author Information
1. Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China
- Publication Type:Research Article
- Keywords:
capsaicin;
breast cancer;
migration and invasion;
silent mating type information regulation 2 homolog 1 (SIRT1);
DNA polymerase δ catalytic subunit p125 encoding gene POLD1 (POLD1);
DNA polymerase δ catalytic subunit p125 (p125)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(7):94-99
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of capsaicin on the migration and invasion of human breast cancer MCF-7 cells and the underlying molecular mechanism. Method: Three capsaicin intervention groups of different concentrations (25, 50, 75 μmol·L-1) and a blank group were set up. After MCF-7 cells were treated with different concentrations of capsaicin (25, 50, 75 μmol·L-1) for 24 h, the cell migration and invasion abilities were assessed by Transwell migration and invasion assay, respectively. Meanwhile, the mRNA level of silent information regulator 2 homolog 1 (SIRT1) and DNA polymerase δ catalytic subunit p125 encoding gene POLD1 (POLD1) were detected by Real-time polymerase chain reaction (Real-time PCR). The protein levels of SIRT1 and DNA polymerase δ catalytic subunit p125 (p125) were detected by Western blot. Result: Compared with the blank group, the number of transmembrane cells was significantly reduced, and the mobility was significantly decreased (P<0.01) in a dose-dependent manner after the intervention of capsaicin (25, 50, 75 μmol·L-1) in MCF-7 cells for 24 h. Capsaicin (25, 50, 75 μmol·L-1) significantly down-regulated the mRNA and protein expressions of SIRT1 (P<0.01) after intervention of capsaicin (25, 50, 75 μmol·L-1) in MCF-7 cells for 24 h. Furthermore, capsaicin (25, 50, 75 μmol·L-1) also significantly down-regulated the mRNA expression of POLD1 and the protein expression of p125 (P<0.01) after intervention of capsaicin (25, 50, 75 μmol·L-1) in MCF-7 cells for 24 h. Conclusion: Capsaicin remarkably inhibits the cell migration and invasion of breast cancer MCF-7 cells, and the possible mechanism may be related to the down-regulation of SIRT1 and POLD1 mRNA expression levels and SIRT1 and p125 protein expression levels.
