Effect of Different Antisepsis Measures on Endogenous Metabolites in Rat Urine
10.13422/j.cnki.syfjx.20182402
- VernacularTitle: 不同防腐措施收集大鼠尿液对其内源性代谢物的影响
- Author:
Huan LIU
1
;
Jie GAO
1
;
Jing-jing YIN
1
;
Li-qun HUANG
1
;
Wen-li XU
1
;
Hui YUAN
1
;
Juan YUE
1
;
Xiu-jun QIN
1
;
Jian-guo LI
1
;
Jun-sheng TIAN
2
Author Information
1. Drug Safety Evaluation Center, Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Key Laboratory of Radiological Toxicology and Preclinical Evaluation of Radiological Drugs in China National Nuclear Corporation, China Institute for Radiation Protection, Taiyuan 030006, China
2. Modern Research Center for Traditional Chinese Medicine(TCM), Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan 030006, China
- Publication Type:Research Article
- Keywords:
urine;
endogenous metabolites;
nuclear magnetic resonance metabolomics;
sodium azide;
ice;
antiseptic measures;
protective conditions
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(6):120-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In the study of urine metabolomics of rats,necessary antiseptic measures should be taken for collection of urine samples,the effect of several antiseptic measures on the endogenous metabolites in urine was studied. Method:The urine samples of rats were collected on ice,sodium azide was added,and both of them were used together to prevent corrosion.Differences of antiseptic measures were analyzed by nuclear magnetic resonance (NMR) metabolomics. Result:The results of NMR metabolomics showed that sodium azide+ice group and ice group had many overlaps,but they clearly separated with the control group and sodium azide group;sodium azide group and the control group had a small part overlap,but there was a tendency of separation.The antiseptic effect of sodium azide+ice group and ice group was similar;compared with control group,valine,betaine and hippuricacid in these two groups increased,but the alanine and 2-ketoglutaric acid decreased. Conclusion:In the study of rat urine metabolomics,low temperature antiseptic measures must be taken when urine samples are collected,and the addition of sodium azide can improve the antiseptic effect slightly under protective conditions.
